<i>BRCA1</i> Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer

Tehseen Hassan; Showkat Ahmad Bhat; Sabhiya Majid; Manzoor R. Mir; Purnima Shrivastava

doi:doi:10.11648/j.crj.20170502.11

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BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer

Tehseen Hassan, Showkat Ahmad Bhat, Sabhiya Majid, Manzoor R. Mir, Purnima Shrivastava

Published in Cancer Research Journal (Volume 5, Issue 2)

Received: 1 May 2017 Accepted: 24 May 2017 Published: 23 June 2017

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Abstract

Background: Breast cancer is the second most common cancer among women after cervical cancer. As Cancer development and progression is dictated by chain of alterations in genes. Over the past few years, the Kashmir valley has witnessed a tremendous increase in the incidence of breast cancer in its unexplored ethnic population. The aim of present study was to find out the role of Promoter Hypermethylation of BRCA1 gene in Breast cancer patients. Material Methods: The DNA was extracted from all the samples and was modified using bisulphite modification kit. Methylation-specific polymerase chain reaction was used for the analysis of the promoter hypermethylation status of BRCA1 gene. Results: The epigenetic analysis revealed that unlike other high risk regions, Kashmiri population has a different promoterhypermethylation profile of BRCA1 gene as 68% of the cases showed BRCA1 promoter hypermethylation in comparison to 20% of the normal cases, the association of promoter hypermethylation with breast cancer and normal cases was found to be significant (P=0.0006). The frequency of BRCA1 promoter hypermethylation was found to be certainly higher in Stage III/IV (75%) compared to Stage I/ II (62%) but the difference was not statistically significant(P =0.0674). The frequency of promoter methylation was foundhigher (77.1%) in age group above 60- years) than ages below 60 years. Conclusion: These results suggest that BRCA1 aberrant promoter hypermethylation in Kashmiri population contributes to the process of carcinogenesis in breast cancer and is reportedly one of the commonest epigenetic changes in the development of breast cancer.

Published in	Cancer Research Journal (Volume 5, Issue 2)
DOI	10.11648/j.crj.20170502.11
Page(s)	9-13
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Breast Cancer, BRCA1, Hypermethylation, Bisulphite Treatment

References

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APA Style

Tehseen Hassan, Showkat Ahmad Bhat, Sabhiya Majid, Manzoor R. Mir, Purnima Shrivastava. (2017). BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Research Journal, 5(2), 9-13. https://doi.org/10.11648/j.crj.20170502.11

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ACS Style

Tehseen Hassan; Showkat Ahmad Bhat; Sabhiya Majid; Manzoor R. Mir; Purnima Shrivastava. BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Res. J. 2017, 5(2), 9-13. doi: 10.11648/j.crj.20170502.11

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AMA Style

Tehseen Hassan, Showkat Ahmad Bhat, Sabhiya Majid, Manzoor R. Mir, Purnima Shrivastava. BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Res J. 2017;5(2):9-13. doi: 10.11648/j.crj.20170502.11

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@article{10.11648/j.crj.20170502.11,
  author = {Tehseen Hassan and Showkat Ahmad Bhat and Sabhiya Majid and Manzoor R. Mir and Purnima Shrivastava},
  title = {BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer},
  journal = {Cancer Research Journal},
  volume = {5},
  number = {2},
  pages = {9-13},
  doi = {10.11648/j.crj.20170502.11},
  url = {https://doi.org/10.11648/j.crj.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20170502.11},
  abstract = {Background: Breast cancer is the second most common cancer among women after cervical cancer. As Cancer development and progression is dictated by chain of alterations in genes. Over the past few years, the Kashmir valley has witnessed a tremendous increase in the incidence of breast cancer in its unexplored ethnic population. The aim of present study was to find out the role of Promoter Hypermethylation of BRCA1 gene in Breast cancer patients. Material Methods: The DNA was extracted from all the samples and was modified using bisulphite modification kit. Methylation-specific polymerase chain reaction was used for the analysis of the promoter hypermethylation status of BRCA1 gene. Results: The epigenetic analysis revealed that unlike other high risk regions, Kashmiri population has a different promoterhypermethylation profile of BRCA1 gene as 68% of the cases showed BRCA1 promoter hypermethylation in comparison to 20% of the normal cases, the association of promoter hypermethylation with breast cancer and normal cases was found to be significant (P=0.0006). The frequency of BRCA1 promoter hypermethylation was found to be certainly higher in Stage III/IV (75%) compared to Stage I/ II (62%) but the difference was not statistically significant(P =0.0674). The frequency of promoter methylation was foundhigher (77.1%) in age group above 60- years) than ages below 60 years. Conclusion: These results suggest that BRCA1 aberrant promoter hypermethylation in Kashmiri population contributes to the process of carcinogenesis in breast cancer and is reportedly one of the commonest epigenetic changes in the development of breast cancer.},
 year = {2017}
}

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TY - JOUR
T1 - BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer
AU - Tehseen Hassan
AU - Showkat Ahmad Bhat
AU - Sabhiya Majid
AU - Manzoor R. Mir
AU - Purnima Shrivastava
Y1 - 2017/06/23
PY - 2017
N1 - https://doi.org/10.11648/j.crj.20170502.11
DO - 10.11648/j.crj.20170502.11
T2 - Cancer Research Journal
JF - Cancer Research Journal
JO - Cancer Research Journal
SP - 9
EP - 13
PB - Science Publishing Group
SN - 2330-8214
UR - https://doi.org/10.11648/j.crj.20170502.11
AB - Background: Breast cancer is the second most common cancer among women after cervical cancer. As Cancer development and progression is dictated by chain of alterations in genes. Over the past few years, the Kashmir valley has witnessed a tremendous increase in the incidence of breast cancer in its unexplored ethnic population. The aim of present study was to find out the role of Promoter Hypermethylation of BRCA1 gene in Breast cancer patients. Material Methods: The DNA was extracted from all the samples and was modified using bisulphite modification kit. Methylation-specific polymerase chain reaction was used for the analysis of the promoter hypermethylation status of BRCA1 gene. Results: The epigenetic analysis revealed that unlike other high risk regions, Kashmiri population has a different promoterhypermethylation profile of BRCA1 gene as 68% of the cases showed BRCA1 promoter hypermethylation in comparison to 20% of the normal cases, the association of promoter hypermethylation with breast cancer and normal cases was found to be significant (P=0.0006). The frequency of BRCA1 promoter hypermethylation was found to be certainly higher in Stage III/IV (75%) compared to Stage I/ II (62%) but the difference was not statistically significant(P =0.0674). The frequency of promoter methylation was foundhigher (77.1%) in age group above 60- years) than ages below 60 years. Conclusion: These results suggest that BRCA1 aberrant promoter hypermethylation in Kashmiri population contributes to the process of carcinogenesis in breast cancer and is reportedly one of the commonest epigenetic changes in the development of breast cancer.
VL - 5
IS - 2
ER -

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Author Information

Tehseen Hassan

Department of Biochemistry, Govt. Medical College Srinagar, Jammu & Kashmir, India
Showkat Ahmad Bhat

Department of Biochemistry, Govt. Medical College Srinagar, Jammu & Kashmir, India
Sabhiya Majid

Department of Biochemistry, Govt. Medical College Srinagar, Jammu & Kashmir, India
Manzoor R. Mir

Division of Vety Biochemistry, Faculty of Veterinary Sciences, SKUAST-K, Jammu & Kashmir, India
Purnima Shrivastava

Director Research Bhagwant University, Sikar Road Ajmer, Rajasthan, India

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APA Style

Tehseen Hassan, Showkat Ahmad Bhat, Sabhiya Majid, Manzoor R. Mir, Purnima Shrivastava. (2017). BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Research Journal, 5(2), 9-13. https://doi.org/10.11648/j.crj.20170502.11

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ACS Style

Tehseen Hassan; Showkat Ahmad Bhat; Sabhiya Majid; Manzoor R. Mir; Purnima Shrivastava. BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Res. J. 2017, 5(2), 9-13. doi: 10.11648/j.crj.20170502.11

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AMA Style

Tehseen Hassan, Showkat Ahmad Bhat, Sabhiya Majid, Manzoor R. Mir, Purnima Shrivastava. BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer. Cancer Res J. 2017;5(2):9-13. doi: 10.11648/j.crj.20170502.11

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@article{10.11648/j.crj.20170502.11,
  author = {Tehseen Hassan and Showkat Ahmad Bhat and Sabhiya Majid and Manzoor R. Mir and Purnima Shrivastava},
  title = {BRCA1 Promoter Hypermethylationas an Early Diagnostic Tool for Breast Cancer},
  journal = {Cancer Research Journal},
  volume = {5},
  number = {2},
  pages = {9-13},
  doi = {10.11648/j.crj.20170502.11},
  url = {https://doi.org/10.11648/j.crj.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20170502.11},
  abstract = {Background: Breast cancer is the second most common cancer among women after cervical cancer. As Cancer development and progression is dictated by chain of alterations in genes. Over the past few years, the Kashmir valley has witnessed a tremendous increase in the incidence of breast cancer in its unexplored ethnic population. The aim of present study was to find out the role of Promoter Hypermethylation of BRCA1 gene in Breast cancer patients. Material Methods: The DNA was extracted from all the samples and was modified using bisulphite modification kit. Methylation-specific polymerase chain reaction was used for the analysis of the promoter hypermethylation status of BRCA1 gene. Results: The epigenetic analysis revealed that unlike other high risk regions, Kashmiri population has a different promoterhypermethylation profile of BRCA1 gene as 68% of the cases showed BRCA1 promoter hypermethylation in comparison to 20% of the normal cases, the association of promoter hypermethylation with breast cancer and normal cases was found to be significant (P=0.0006). The frequency of BRCA1 promoter hypermethylation was found to be certainly higher in Stage III/IV (75%) compared to Stage I/ II (62%) but the difference was not statistically significant(P =0.0674). The frequency of promoter methylation was foundhigher (77.1%) in age group above 60- years) than ages below 60 years. Conclusion: These results suggest that BRCA1 aberrant promoter hypermethylation in Kashmiri population contributes to the process of carcinogenesis in breast cancer and is reportedly one of the commonest epigenetic changes in the development of breast cancer.},
 year = {2017}
}

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