Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechanism. Methods: Quantitative real-time PCR with reverse transcription (RT-qPCR) was employed to determine the expression level of miR-218-5p that trying to investigate if there is a link to clinicopathological characteristics. HCT116 cells were transfected with lentivirus for stably expressing miR-218-5p which was validated, as well as its target gene CDH2, by RT-qPCR and Western blotting. To generate xenograft models, BALB/c nude mice were treated with transfected cells for stably overexpressing miR-218-5p. Finally, the lung metastasis of CRC was observed. Results: The miR-218-5p expression was significantly downregulated in CRC tissues, playing the role of indicator for lymph node metastasis, advanced clinical stage, and poor prognosis. In addition, the expression level of CDH2 was negatively related to miR-218-5p in lentiviral transfected HCT116 cells. At the same time, overexpressed miR-218-5p inhibited CRC cell xenografts metastasis. Conclusion: Data indicates that miR-218-5p suppresses the metastasis of CRC cells, in part by inhibiting CDH2.
| Published in | Cancer Research Journal (Volume 11, Issue 3) |
| DOI | 10.11648/j.crj.20231103.11 |
| Page(s) | 86-90 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Colorectal Cancer, CDH2, miR-218-5p
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APA Style
Hailong Zhu, Xuyou Zhu, Xiaoli Li, Ran Cui. (2023). Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer. Cancer Research Journal, 11(3), 86-90. https://doi.org/10.11648/j.crj.20231103.11
ACS Style
Hailong Zhu; Xuyou Zhu; Xiaoli Li; Ran Cui. Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer. Cancer Res. J. 2023, 11(3), 86-90. doi: 10.11648/j.crj.20231103.11
AMA Style
Hailong Zhu, Xuyou Zhu, Xiaoli Li, Ran Cui. Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer. Cancer Res J. 2023;11(3):86-90. doi: 10.11648/j.crj.20231103.11
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Download@article{10.11648/j.crj.20231103.11,
author = {Hailong Zhu and Xuyou Zhu and Xiaoli Li and Ran Cui},
title = {Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer},
journal = {Cancer Research Journal},
volume = {11},
number = {3},
pages = {86-90},
doi = {10.11648/j.crj.20231103.11},
url = {https://doi.org/10.11648/j.crj.20231103.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20231103.11},
abstract = {Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechanism. Methods: Quantitative real-time PCR with reverse transcription (RT-qPCR) was employed to determine the expression level of miR-218-5p that trying to investigate if there is a link to clinicopathological characteristics. HCT116 cells were transfected with lentivirus for stably expressing miR-218-5p which was validated, as well as its target gene CDH2, by RT-qPCR and Western blotting. To generate xenograft models, BALB/c nude mice were treated with transfected cells for stably overexpressing miR-218-5p. Finally, the lung metastasis of CRC was observed. Results: The miR-218-5p expression was significantly downregulated in CRC tissues, playing the role of indicator for lymph node metastasis, advanced clinical stage, and poor prognosis. In addition, the expression level of CDH2 was negatively related to miR-218-5p in lentiviral transfected HCT116 cells. At the same time, overexpressed miR-218-5p inhibited CRC cell xenografts metastasis. Conclusion: Data indicates that miR-218-5p suppresses the metastasis of CRC cells, in part by inhibiting CDH2.},
year = {2023}
}
TY - JOUR T1 - Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer AU - Hailong Zhu AU - Xuyou Zhu AU - Xiaoli Li AU - Ran Cui Y1 - 2023/07/06 PY - 2023 N1 - https://doi.org/10.11648/j.crj.20231103.11 DO - 10.11648/j.crj.20231103.11 T2 - Cancer Research Journal JF - Cancer Research Journal JO - Cancer Research Journal SP - 86 EP - 90 PB - Science Publishing Group SN - 2330-8214 UR - https://doi.org/10.11648/j.crj.20231103.11 AB - Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechanism. Methods: Quantitative real-time PCR with reverse transcription (RT-qPCR) was employed to determine the expression level of miR-218-5p that trying to investigate if there is a link to clinicopathological characteristics. HCT116 cells were transfected with lentivirus for stably expressing miR-218-5p which was validated, as well as its target gene CDH2, by RT-qPCR and Western blotting. To generate xenograft models, BALB/c nude mice were treated with transfected cells for stably overexpressing miR-218-5p. Finally, the lung metastasis of CRC was observed. Results: The miR-218-5p expression was significantly downregulated in CRC tissues, playing the role of indicator for lymph node metastasis, advanced clinical stage, and poor prognosis. In addition, the expression level of CDH2 was negatively related to miR-218-5p in lentiviral transfected HCT116 cells. At the same time, overexpressed miR-218-5p inhibited CRC cell xenografts metastasis. Conclusion: Data indicates that miR-218-5p suppresses the metastasis of CRC cells, in part by inhibiting CDH2. VL - 11 IS - 3 ER -
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