In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats

Issiyakou Haman; Ahmadou Adamou; Francis Nveikoueing; Nicolas Yanou Njintang; Dieudonné Ndjonka

doi:doi:10.11648/j.jdmp.20210704.11

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In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats

Issiyakou Haman, Ahmadou Adamou, Francis Nveikoueing, Nicolas Yanou Njintang, Dieudonné Ndjonka

Published in Journal of Diseases and Medicinal Plants (Volume 7, Issue 4)

Received: 28 August 2021 Accepted: 26 September 2021 Published: 12 October 2021

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Abstract

In Cameroon, the practice of traditional medicine in the treatment of intestinal helminthiasis is a reality and habits in the lives of traditional healers and breeders for various reasons related to their local life situations. In the valorization of medicinal plants in the treatment of digestive parasitosis as an alternative to synthetic anthelmintics, a study was carried out in order to verify their anthelmintic activity and the level of their toxicity. The present study was carried out to determine the in vitro anthelminthic activity of Tephrosia pedicellata extracts. Aqueous and hydroethanolic leaves extracts at different concentrations were tested against Haemonchus contortus development stages, mutant and wild type of free-living nematode Caenorhabditis elegans. The two plant extracts were then chemically screened and their toxicological profile was established using albino rats. Hydroethanolic extract was the most potent by killing Haemonchus contortus adults (LC₅₀: 0.038 mg/mL after 24 hours), infesting larvae (LC₅₀: 0.22 mg/mL after 48 hours) and inhibiting egg hatching (IC₅₀: 0.76 mg/mL after 48 hours). The two plant extracts showed same activity on Caenorhabditis elegans levamisole non-sensitive (LC₅₀: 0.26 mg/mL after 48 hours) and sensitive strains (LC₅₀: 0.25 mg/mL after 48 hours), showing different mode of action to that of levamisole. Several plant chemicals were identified from both extracts, with higher content of polyphenol compounds (562.91 mg GAE/g DW), flavonoids (135.96 mg RE/g DW), condensed tannins (228.50 mg CE/g DW) and saponins (206.83 mg SE/g DW) obtained with the hydroethanolic extract. No toxic effect was observed during acute toxicity study. Moreover, apart from the significant increase of platelets and Aspartate transaminase (p=0.001) and the glomerular inflammation in the lungs at the dose of 1000 mg/kg, no harmful variation of haematological and biochemical parameters of rats were observed. The pattern of anthelmintic activity of these extracts on Caenorhabditis elegans and the non-toxic effect of hydroethanolic extract provide a way for new anthelminthic drug.

Published in	Journal of Diseases and Medicinal Plants (Volume 7, Issue 4)
DOI	10.11648/j.jdmp.20210704.11
Page(s)	87-97
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Haemonchus contortus, Caenorhabditis elegans, Tephrosia pedicellata, Plant Chemicals, Anthelminthic, Toxicity

References

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Issiyakou Haman, Ahmadou Adamou, Francis Nveikoueing, Nicolas Yanou Njintang, Dieudonné Ndjonka. (2021). In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats. Journal of Diseases and Medicinal Plants, 7(4), 87-97. https://doi.org/10.11648/j.jdmp.20210704.11

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Issiyakou Haman; Ahmadou Adamou; Francis Nveikoueing; Nicolas Yanou Njintang; Dieudonné Ndjonka. In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats. J. Dis. Med. Plants 2021, 7(4), 87-97. doi: 10.11648/j.jdmp.20210704.11

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Issiyakou Haman, Ahmadou Adamou, Francis Nveikoueing, Nicolas Yanou Njintang, Dieudonné Ndjonka. In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats. J Dis Med Plants. 2021;7(4):87-97. doi: 10.11648/j.jdmp.20210704.11

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@article{10.11648/j.jdmp.20210704.11,
  author = {Issiyakou Haman and Ahmadou Adamou and Francis Nveikoueing and Nicolas Yanou Njintang and Dieudonné Ndjonka},
  title = {In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {7},
  number = {4},
  pages = {87-97},
  doi = {10.11648/j.jdmp.20210704.11},
  url = {https://doi.org/10.11648/j.jdmp.20210704.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20210704.11},
  abstract = {In Cameroon, the practice of traditional medicine in the treatment of intestinal helminthiasis is a reality and habits in the lives of traditional healers and breeders for various reasons related to their local life situations. In the valorization of medicinal plants in the treatment of digestive parasitosis as an alternative to synthetic anthelmintics, a study was carried out in order to verify their anthelmintic activity and the level of their toxicity. The present study was carried out to determine the in vitro anthelminthic activity of Tephrosia pedicellata extracts. Aqueous and hydroethanolic leaves extracts at different concentrations were tested against Haemonchus contortus development stages, mutant and wild type of free-living nematode Caenorhabditis elegans. The two plant extracts were then chemically screened and their toxicological profile was established using albino rats. Hydroethanolic extract was the most potent by killing Haemonchus contortus adults (LC50: 0.038 mg/mL after 24 hours), infesting larvae (LC50: 0.22 mg/mL after 48 hours) and inhibiting egg hatching (IC50: 0.76 mg/mL after 48 hours). The two plant extracts showed same activity on Caenorhabditis elegans levamisole non-sensitive (LC50: 0.26 mg/mL after 48 hours) and sensitive strains (LC50: 0.25 mg/mL after 48 hours), showing different mode of action to that of levamisole. Several plant chemicals were identified from both extracts, with higher content of polyphenol compounds (562.91 mg GAE/g DW), flavonoids (135.96 mg RE/g DW), condensed tannins (228.50 mg CE/g DW) and saponins (206.83 mg SE/g DW) obtained with the hydroethanolic extract. No toxic effect was observed during acute toxicity study. Moreover, apart from the significant increase of platelets and Aspartate transaminase (p=0.001) and the glomerular inflammation in the lungs at the dose of 1000 mg/kg, no harmful variation of haematological and biochemical parameters of rats were observed. The pattern of anthelmintic activity of these extracts on Caenorhabditis elegans and the non-toxic effect of hydroethanolic extract provide a way for new anthelminthic drug.},
 year = {2021}
}

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TY  - JOUR
T1  - In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats
AU  - Issiyakou Haman
AU  - Ahmadou Adamou
AU  - Francis Nveikoueing
AU  - Nicolas Yanou Njintang
AU  - Dieudonné Ndjonka
Y1  - 2021/10/12
PY  - 2021
N1  - https://doi.org/10.11648/j.jdmp.20210704.11
DO  - 10.11648/j.jdmp.20210704.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 87
EP  - 97
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20210704.11
AB  - In Cameroon, the practice of traditional medicine in the treatment of intestinal helminthiasis is a reality and habits in the lives of traditional healers and breeders for various reasons related to their local life situations. In the valorization of medicinal plants in the treatment of digestive parasitosis as an alternative to synthetic anthelmintics, a study was carried out in order to verify their anthelmintic activity and the level of their toxicity. The present study was carried out to determine the in vitro anthelminthic activity of Tephrosia pedicellata extracts. Aqueous and hydroethanolic leaves extracts at different concentrations were tested against Haemonchus contortus development stages, mutant and wild type of free-living nematode Caenorhabditis elegans. The two plant extracts were then chemically screened and their toxicological profile was established using albino rats. Hydroethanolic extract was the most potent by killing Haemonchus contortus adults (LC50: 0.038 mg/mL after 24 hours), infesting larvae (LC50: 0.22 mg/mL after 48 hours) and inhibiting egg hatching (IC50: 0.76 mg/mL after 48 hours). The two plant extracts showed same activity on Caenorhabditis elegans levamisole non-sensitive (LC50: 0.26 mg/mL after 48 hours) and sensitive strains (LC50: 0.25 mg/mL after 48 hours), showing different mode of action to that of levamisole. Several plant chemicals were identified from both extracts, with higher content of polyphenol compounds (562.91 mg GAE/g DW), flavonoids (135.96 mg RE/g DW), condensed tannins (228.50 mg CE/g DW) and saponins (206.83 mg SE/g DW) obtained with the hydroethanolic extract. No toxic effect was observed during acute toxicity study. Moreover, apart from the significant increase of platelets and Aspartate transaminase (p=0.001) and the glomerular inflammation in the lungs at the dose of 1000 mg/kg, no harmful variation of haematological and biochemical parameters of rats were observed. The pattern of anthelmintic activity of these extracts on Caenorhabditis elegans and the non-toxic effect of hydroethanolic extract provide a way for new anthelminthic drug.
VL  - 7
IS  - 4
ER  -

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Author Information

Issiyakou Haman

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Ahmadou Adamou

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Francis Nveikoueing

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Nicolas Yanou Njintang

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Dieudonné Ndjonka

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon

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Issiyakou Haman, Ahmadou Adamou, Francis Nveikoueing, Nicolas Yanou Njintang, Dieudonné Ndjonka. (2021). In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats. Journal of Diseases and Medicinal Plants, 7(4), 87-97. https://doi.org/10.11648/j.jdmp.20210704.11

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Issiyakou Haman; Ahmadou Adamou; Francis Nveikoueing; Nicolas Yanou Njintang; Dieudonné Ndjonka. In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats. J. Dis. Med. Plants 2021, 7(4), 87-97. doi: 10.11648/j.jdmp.20210704.11

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Issiyakou Haman, Ahmadou Adamou, Francis Nveikoueing, Nicolas Yanou Njintang, Dieudonné Ndjonka. In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats. J Dis Med Plants. 2021;7(4):87-97. doi: 10.11648/j.jdmp.20210704.11

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@article{10.11648/j.jdmp.20210704.11,
  author = {Issiyakou Haman and Ahmadou Adamou and Francis Nveikoueing and Nicolas Yanou Njintang and Dieudonné Ndjonka},
  title = {In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {7},
  number = {4},
  pages = {87-97},
  doi = {10.11648/j.jdmp.20210704.11},
  url = {https://doi.org/10.11648/j.jdmp.20210704.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20210704.11},
  abstract = {In Cameroon, the practice of traditional medicine in the treatment of intestinal helminthiasis is a reality and habits in the lives of traditional healers and breeders for various reasons related to their local life situations. In the valorization of medicinal plants in the treatment of digestive parasitosis as an alternative to synthetic anthelmintics, a study was carried out in order to verify their anthelmintic activity and the level of their toxicity. The present study was carried out to determine the in vitro anthelminthic activity of Tephrosia pedicellata extracts. Aqueous and hydroethanolic leaves extracts at different concentrations were tested against Haemonchus contortus development stages, mutant and wild type of free-living nematode Caenorhabditis elegans. The two plant extracts were then chemically screened and their toxicological profile was established using albino rats. Hydroethanolic extract was the most potent by killing Haemonchus contortus adults (LC50: 0.038 mg/mL after 24 hours), infesting larvae (LC50: 0.22 mg/mL after 48 hours) and inhibiting egg hatching (IC50: 0.76 mg/mL after 48 hours). The two plant extracts showed same activity on Caenorhabditis elegans levamisole non-sensitive (LC50: 0.26 mg/mL after 48 hours) and sensitive strains (LC50: 0.25 mg/mL after 48 hours), showing different mode of action to that of levamisole. Several plant chemicals were identified from both extracts, with higher content of polyphenol compounds (562.91 mg GAE/g DW), flavonoids (135.96 mg RE/g DW), condensed tannins (228.50 mg CE/g DW) and saponins (206.83 mg SE/g DW) obtained with the hydroethanolic extract. No toxic effect was observed during acute toxicity study. Moreover, apart from the significant increase of platelets and Aspartate transaminase (p=0.001) and the glomerular inflammation in the lungs at the dose of 1000 mg/kg, no harmful variation of haematological and biochemical parameters of rats were observed. The pattern of anthelmintic activity of these extracts on Caenorhabditis elegans and the non-toxic effect of hydroethanolic extract provide a way for new anthelminthic drug.},
 year = {2021}
}

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TY  - JOUR
T1  - In vitro Anthelmintic Activity of Tephrosia pedicellata on Two Nematodes (Haemonchus contortus, Caenorhaditis elegans) and Its In vivo Toxicity on Rats
AU  - Issiyakou Haman
AU  - Ahmadou Adamou
AU  - Francis Nveikoueing
AU  - Nicolas Yanou Njintang
AU  - Dieudonné Ndjonka
Y1  - 2021/10/12
PY  - 2021
N1  - https://doi.org/10.11648/j.jdmp.20210704.11
DO  - 10.11648/j.jdmp.20210704.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 87
EP  - 97
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20210704.11
AB  - In Cameroon, the practice of traditional medicine in the treatment of intestinal helminthiasis is a reality and habits in the lives of traditional healers and breeders for various reasons related to their local life situations. In the valorization of medicinal plants in the treatment of digestive parasitosis as an alternative to synthetic anthelmintics, a study was carried out in order to verify their anthelmintic activity and the level of their toxicity. The present study was carried out to determine the in vitro anthelminthic activity of Tephrosia pedicellata extracts. Aqueous and hydroethanolic leaves extracts at different concentrations were tested against Haemonchus contortus development stages, mutant and wild type of free-living nematode Caenorhabditis elegans. The two plant extracts were then chemically screened and their toxicological profile was established using albino rats. Hydroethanolic extract was the most potent by killing Haemonchus contortus adults (LC50: 0.038 mg/mL after 24 hours), infesting larvae (LC50: 0.22 mg/mL after 48 hours) and inhibiting egg hatching (IC50: 0.76 mg/mL after 48 hours). The two plant extracts showed same activity on Caenorhabditis elegans levamisole non-sensitive (LC50: 0.26 mg/mL after 48 hours) and sensitive strains (LC50: 0.25 mg/mL after 48 hours), showing different mode of action to that of levamisole. Several plant chemicals were identified from both extracts, with higher content of polyphenol compounds (562.91 mg GAE/g DW), flavonoids (135.96 mg RE/g DW), condensed tannins (228.50 mg CE/g DW) and saponins (206.83 mg SE/g DW) obtained with the hydroethanolic extract. No toxic effect was observed during acute toxicity study. Moreover, apart from the significant increase of platelets and Aspartate transaminase (p=0.001) and the glomerular inflammation in the lungs at the dose of 1000 mg/kg, no harmful variation of haematological and biochemical parameters of rats were observed. The pattern of anthelmintic activity of these extracts on Caenorhabditis elegans and the non-toxic effect of hydroethanolic extract provide a way for new anthelminthic drug.
VL  - 7
IS  - 4
ER  -

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