Display of Glucose Oxidase on <i>Bacillus subtilis</i> Spore Surface and Its Application in Electrode

Wenhu Chen; Runlong Ma; Ping Lin; Haibo Yuan; Hongling Liu; Yi Jiang; Di Huang; Yaohong Ma; Tengfei Wang

doi:doi:10.11648/j.wjac.20220702.11

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Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode

Wenhu Chen, Runlong Ma, Ping Lin, Haibo Yuan, Hongling Liu, Yi Jiang, Di Huang, Yaohong Ma, Tengfei Wang

Published in World Journal of Applied Chemistry (Volume 7, Issue 2)

Received: 12 March 2022 Accepted: 30 March 2022 Published: 8 April 2022

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Abstract

Glucose oxidase (GOD) is widely used in the fields of food, chemistry and medicine due to its specific catalytic activity, and it is the main tool enzyme in the biological field. The biological preparation of GOD can be realized by the method of surface display on Bacillus subtilis spores. In this study, we analyzed the display characteristics of eleven cot proteins on the spore coat and determined the fluorescence intensity of different spore coat proteins by flow cytometry as follows: CotX > CotY > CotF > CotC > CotE > CotG > CotV > CotB > CotM > CotA > CotW. Next, CotC, CotX and CotY were selected to display GOD on the surface of spores, and CotX demonstrated the highest GOD activity. Subsequently, graphene oxide was added to the glassy carbon electrode and prussian blue was deposited, the surface of this electrode was fixed with spore–GOD and finally covered with a layer of Nafion solution to prepare glucose detection electrode. In the range of 0.1-7.0 mmol/L glucose, the cyclic voltammetry curve on the enzyme electrode showed a linear relationship with the glucose concentration. The calibration curve equation was I = 1.3047C_glucose+ 3.639 (R² = 0.9929), and its detection limit was 7.5 μmol/L (S/N = 3). This modified electrode has good conductivity, stability, reproducibility and can be used for the analysis and determination of glucose.

Published in	World Journal of Applied Chemistry (Volume 7, Issue 2)
DOI	10.11648/j.wjac.20220702.11
Page(s)	34-47
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Bacillus subtilis, Spores, Coat Protein, Surface Display, Glucose Oxidase, Enzyme Electrode

References

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APA Style

Wenhu Chen, Runlong Ma, Ping Lin, Haibo Yuan, Hongling Liu, et al. (2022). Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode. World Journal of Applied Chemistry, 7(2), 34-47. https://doi.org/10.11648/j.wjac.20220702.11

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ACS Style

Wenhu Chen; Runlong Ma; Ping Lin; Haibo Yuan; Hongling Liu, et al. Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode. World J. Appl. Chem. 2022, 7(2), 34-47. doi: 10.11648/j.wjac.20220702.11

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AMA Style

Wenhu Chen, Runlong Ma, Ping Lin, Haibo Yuan, Hongling Liu, et al. Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode. World J Appl Chem. 2022;7(2):34-47. doi: 10.11648/j.wjac.20220702.11

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@article{10.11648/j.wjac.20220702.11,
  author = {Wenhu Chen and Runlong Ma and Ping Lin and Haibo Yuan and Hongling Liu and Yi Jiang and Di Huang and Yaohong Ma and Tengfei Wang},
  title = {Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode},
  journal = {World Journal of Applied Chemistry},
  volume = {7},
  number = {2},
  pages = {34-47},
  doi = {10.11648/j.wjac.20220702.11},
  url = {https://doi.org/10.11648/j.wjac.20220702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjac.20220702.11},
  abstract = {Glucose oxidase (GOD) is widely used in the fields of food, chemistry and medicine due to its specific catalytic activity, and it is the main tool enzyme in the biological field. The biological preparation of GOD can be realized by the method of surface display on Bacillus subtilis spores. In this study, we analyzed the display characteristics of eleven cot proteins on the spore coat and determined the fluorescence intensity of different spore coat proteins by flow cytometry as follows: CotX > CotY > CotF > CotC > CotE > CotG > CotV > CotB > CotM > CotA > CotW. Next, CotC, CotX and CotY were selected to display GOD on the surface of spores, and CotX demonstrated the highest GOD activity. Subsequently, graphene oxide was added to the glassy carbon electrode and prussian blue was deposited, the surface of this electrode was fixed with spore–GOD and finally covered with a layer of Nafion solution to prepare glucose detection electrode. In the range of 0.1-7.0 mmol/L glucose, the cyclic voltammetry curve on the enzyme electrode showed a linear relationship with the glucose concentration. The calibration curve equation was I = 1.3047Cglucose + 3.639 (R2 = 0.9929), and its detection limit was 7.5 μmol/L (S/N = 3). This modified electrode has good conductivity, stability, reproducibility and can be used for the analysis and determination of glucose.},
 year = {2022}
}

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TY  - JOUR
T1  - Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode
AU  - Wenhu Chen
AU  - Runlong Ma
AU  - Ping Lin
AU  - Haibo Yuan
AU  - Hongling Liu
AU  - Yi Jiang
AU  - Di Huang
AU  - Yaohong Ma
AU  - Tengfei Wang
Y1  - 2022/04/08
PY  - 2022
N1  - https://doi.org/10.11648/j.wjac.20220702.11
DO  - 10.11648/j.wjac.20220702.11
T2  - World Journal of Applied Chemistry
JF  - World Journal of Applied Chemistry
JO  - World Journal of Applied Chemistry
SP  - 34
EP  - 47
PB  - Science Publishing Group
SN  - 2637-5982
UR  - https://doi.org/10.11648/j.wjac.20220702.11
AB  - Glucose oxidase (GOD) is widely used in the fields of food, chemistry and medicine due to its specific catalytic activity, and it is the main tool enzyme in the biological field. The biological preparation of GOD can be realized by the method of surface display on Bacillus subtilis spores. In this study, we analyzed the display characteristics of eleven cot proteins on the spore coat and determined the fluorescence intensity of different spore coat proteins by flow cytometry as follows: CotX > CotY > CotF > CotC > CotE > CotG > CotV > CotB > CotM > CotA > CotW. Next, CotC, CotX and CotY were selected to display GOD on the surface of spores, and CotX demonstrated the highest GOD activity. Subsequently, graphene oxide was added to the glassy carbon electrode and prussian blue was deposited, the surface of this electrode was fixed with spore–GOD and finally covered with a layer of Nafion solution to prepare glucose detection electrode. In the range of 0.1-7.0 mmol/L glucose, the cyclic voltammetry curve on the enzyme electrode showed a linear relationship with the glucose concentration. The calibration curve equation was I = 1.3047Cglucose + 3.639 (R2 = 0.9929), and its detection limit was 7.5 μmol/L (S/N = 3). This modified electrode has good conductivity, stability, reproducibility and can be used for the analysis and determination of glucose.
VL  - 7
IS  - 2
ER  -

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Author Information

Wenhu Chen

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China
Runlong Ma

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China
Ping Lin

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China
Haibo Yuan

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China
Hongling Liu

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China
Yi Jiang

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China
Di Huang

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China
Yaohong Ma

Shandong Key Laboratory of Biosensor, Biology Institute of Shandong Academy of Sciences, Jinan, People’s Republic of China
Tengfei Wang

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China

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APA Style

Wenhu Chen, Runlong Ma, Ping Lin, Haibo Yuan, Hongling Liu, et al. (2022). Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode. World Journal of Applied Chemistry, 7(2), 34-47. https://doi.org/10.11648/j.wjac.20220702.11

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ACS Style

Wenhu Chen; Runlong Ma; Ping Lin; Haibo Yuan; Hongling Liu, et al. Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode. World J. Appl. Chem. 2022, 7(2), 34-47. doi: 10.11648/j.wjac.20220702.11

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AMA Style

Wenhu Chen, Runlong Ma, Ping Lin, Haibo Yuan, Hongling Liu, et al. Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode. World J Appl Chem. 2022;7(2):34-47. doi: 10.11648/j.wjac.20220702.11

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@article{10.11648/j.wjac.20220702.11,
  author = {Wenhu Chen and Runlong Ma and Ping Lin and Haibo Yuan and Hongling Liu and Yi Jiang and Di Huang and Yaohong Ma and Tengfei Wang},
  title = {Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode},
  journal = {World Journal of Applied Chemistry},
  volume = {7},
  number = {2},
  pages = {34-47},
  doi = {10.11648/j.wjac.20220702.11},
  url = {https://doi.org/10.11648/j.wjac.20220702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjac.20220702.11},
  abstract = {Glucose oxidase (GOD) is widely used in the fields of food, chemistry and medicine due to its specific catalytic activity, and it is the main tool enzyme in the biological field. The biological preparation of GOD can be realized by the method of surface display on Bacillus subtilis spores. In this study, we analyzed the display characteristics of eleven cot proteins on the spore coat and determined the fluorescence intensity of different spore coat proteins by flow cytometry as follows: CotX > CotY > CotF > CotC > CotE > CotG > CotV > CotB > CotM > CotA > CotW. Next, CotC, CotX and CotY were selected to display GOD on the surface of spores, and CotX demonstrated the highest GOD activity. Subsequently, graphene oxide was added to the glassy carbon electrode and prussian blue was deposited, the surface of this electrode was fixed with spore–GOD and finally covered with a layer of Nafion solution to prepare glucose detection electrode. In the range of 0.1-7.0 mmol/L glucose, the cyclic voltammetry curve on the enzyme electrode showed a linear relationship with the glucose concentration. The calibration curve equation was I = 1.3047Cglucose + 3.639 (R2 = 0.9929), and its detection limit was 7.5 μmol/L (S/N = 3). This modified electrode has good conductivity, stability, reproducibility and can be used for the analysis and determination of glucose.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Display of Glucose Oxidase on Bacillus subtilis Spore Surface and Its Application in Electrode
AU  - Wenhu Chen
AU  - Runlong Ma
AU  - Ping Lin
AU  - Haibo Yuan
AU  - Hongling Liu
AU  - Yi Jiang
AU  - Di Huang
AU  - Yaohong Ma
AU  - Tengfei Wang
Y1  - 2022/04/08
PY  - 2022
N1  - https://doi.org/10.11648/j.wjac.20220702.11
DO  - 10.11648/j.wjac.20220702.11
T2  - World Journal of Applied Chemistry
JF  - World Journal of Applied Chemistry
JO  - World Journal of Applied Chemistry
SP  - 34
EP  - 47
PB  - Science Publishing Group
SN  - 2637-5982
UR  - https://doi.org/10.11648/j.wjac.20220702.11
AB  - Glucose oxidase (GOD) is widely used in the fields of food, chemistry and medicine due to its specific catalytic activity, and it is the main tool enzyme in the biological field. The biological preparation of GOD can be realized by the method of surface display on Bacillus subtilis spores. In this study, we analyzed the display characteristics of eleven cot proteins on the spore coat and determined the fluorescence intensity of different spore coat proteins by flow cytometry as follows: CotX > CotY > CotF > CotC > CotE > CotG > CotV > CotB > CotM > CotA > CotW. Next, CotC, CotX and CotY were selected to display GOD on the surface of spores, and CotX demonstrated the highest GOD activity. Subsequently, graphene oxide was added to the glassy carbon electrode and prussian blue was deposited, the surface of this electrode was fixed with spore–GOD and finally covered with a layer of Nafion solution to prepare glucose detection electrode. In the range of 0.1-7.0 mmol/L glucose, the cyclic voltammetry curve on the enzyme electrode showed a linear relationship with the glucose concentration. The calibration curve equation was I = 1.3047Cglucose + 3.639 (R2 = 0.9929), and its detection limit was 7.5 μmol/L (S/N = 3). This modified electrode has good conductivity, stability, reproducibility and can be used for the analysis and determination of glucose.
VL  - 7
IS  - 2
ER  -

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