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Research Article |

Effect of 3–3'diindolylmethane (DIM) on Acute Radiation Lung Injury in Mice Via the TGF-β1 Pathway

Objective: We studied the effect of 3–3'diindole methane (DIM) on acute radiation-induced lung injury (RILI) in mice and the possible underlying mechanism. Methods: A total of 45 mice were divided into five groups using the random number table method, namely, blank group, drug group alone (75 mg/kg DIM by peritoneal perfusion), simple irradiation group (one-time irradiation of 16 Gy), irradiation + drug group (one-time irradiation of 16 Gy + intraperitoneal perfusion of 75 mg/kg DIM 30 min before irradiation), and irradiation + prednisone group (one-time irradiation of 16 Gy + intraperitoneal perfusion of 5 mg/kg prednisone 30 min before irradiationas a positive control group). The whole lung was irradiated with a single dose of 16 Gy X-ray. Mice were killed by cervical dislocation at 24 h, 1 week, 2 weeks, and 4 weeks after irradiation, following which the lung tissue samples were subjected to hematoxylin and eosin (HE) staining. In addition, the expression of transforming growth factor (TGF)-β1/vascular endothelial growth factor (VEGF) pathway-related proteins was studied. Results: Under the same irradiation dose, the degree of lung injury in model mice after the intervention of DIM drugs was significantly lower than that in mice in the simple irradiation group. DIM significantly reduced the expression of TGF-β/VEGF-1 (P< 0.05). Conclusion: 3-3'diindolyl methane (DIM) can be downregulated by TGF-β1 signaling pathway, thereby reducing the expression of VEGF in lung tissue, inhibiting radiation-induced oxidative stress and inflammatory factor release in mouse lung tissue, and reducing the degree of RILI. These prospective experimental research results provide necessary experimental and theoretical basis for the application of 3-3'diindolemethane and its derivatives as new radiation protective drugs in clinical tumor radiotherapy.

3–3'diindolylmethane (DIM), TGF-β1/VEGF Pathway, Mice, Radiotherapy, Radiation-Induced Lung Injury

Qin Ge. (2023). Effect of 3–3'diindolylmethane (DIM) on Acute Radiation Lung Injury in Mice Via the TGF-β1 Pathway. American Journal of Clinical and Experimental Medicine, 11(4), 66-72.

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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