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Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology

In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.

SDS-PAGE Gel Electrophoresis, Milk, Soybean Protein, Coconut Milk, Protein Characteristic Bands

Zhang Yushan, Li Biyi, Shen Zhihua, Liu Yonggang. (2023). Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Science Discovery, 11(6), 189-192. https://doi.org/10.11648/j.sd.20231106.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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