Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid

Zhengyi Zhou; Zhuo Tang; Zihao Dai; Shuhe Xu; Yujie Ge; Yuemin Ding; Xiong Zhang

doi:doi:10.11648/j.bio.20251306.11

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Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid

Zhengyi Zhou, Zhuo Tang, Zihao Dai, Shuhe Xu, Yujie Ge, Yuemin Ding^*

, Xiong Zhang

Published in American Journal of Bioscience and Bioengineering (Volume 13, Issue 6)

Received: 21 November 2025 Accepted: 21 November 2025 Published: 22 November 2025

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Abstract

This study comprehensively utilized toad hearts and myocardial cell models to explore the effects of acute lead exposure on myocardial contractile function and the therapeutic effect of alpha lipoic acid (ALA). The results showed that lead acetate (PbAc) dose dependently inhibited the contractile function of toad hearts. Acute treatment with 50, 150, and 450 mM PbAc reduced the amplitude of heart contraction to 92.8%, 78.0%, and 69.7% of the control before medication (P<0.01), respectively, and reduced heart rate to 92.4%, 92.5%, and 54.9% of the control before medication (P<0.05). After treatment with a middle concentration of PbAc (150 mM) for 24 hours, the cell viability of H9c2 decreased to 71.9% of the control group (P<0.05). Compared with the lead exposure group, ALA at a concentration of 10 μM significantly improved cardiac contractile function, increasing the amplitude of cardiac contractions and heart rate to 109.4% and 134.2% of the control, respectively (P<0.05). Correspondingly, ALA at a concentration of 20 μM increased the cell viability of H9c2 to 112.6% of the control group (P<0.05). This study indicates that PbAc can significantly inhibit myocardial contractility and reduce cell viability, while ALA can antagonize lead induced myocardial injury and has significant cardioprotective potential.

Published in	American Journal of Bioscience and Bioengineering (Volume 13, Issue 6)
DOI	10.11648/j.bio.20251306.11
Page(s)	106-110
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

Lead Exposure, Myocardial Cells, Cardiac Contractility, Heart Rate, Alpha Lipoic Acid

References

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APA Style

Zhou, Z., Tang, Z., Dai, Z., Xu, S., Ge, Y., et al. (2025). Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid. American Journal of Bioscience and Bioengineering, 13(6), 106-110. https://doi.org/10.11648/j.bio.20251306.11

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ACS Style

Zhou, Z.; Tang, Z.; Dai, Z.; Xu, S.; Ge, Y., et al. Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid. Am. J. BioSci. Bioeng. 2025, 13(6), 106-110. doi: 10.11648/j.bio.20251306.11

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AMA Style

Zhou Z, Tang Z, Dai Z, Xu S, Ge Y, et al. Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid. Am J BioSci Bioeng. 2025;13(6):106-110. doi: 10.11648/j.bio.20251306.11

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@article{10.11648/j.bio.20251306.11,
  author = {Zhengyi Zhou and Zhuo Tang and Zihao Dai and Shuhe Xu and Yujie Ge and Yuemin Ding and Xiong Zhang},
  title = {Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid
},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {13},
  number = {6},
  pages = {106-110},
  doi = {10.11648/j.bio.20251306.11},
  url = {https://doi.org/10.11648/j.bio.20251306.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20251306.11},
  abstract = {This study comprehensively utilized toad hearts and myocardial cell models to explore the effects of acute lead exposure on myocardial contractile function and the therapeutic effect of alpha lipoic acid (ALA). The results showed that lead acetate (PbAc) dose dependently inhibited the contractile function of toad hearts. Acute treatment with 50, 150, and 450 mM PbAc reduced the amplitude of heart contraction to 92.8%, 78.0%, and 69.7% of the control before medication (PPPPP<0.05). This study indicates that PbAc can significantly inhibit myocardial contractility and reduce cell viability, while ALA can antagonize lead induced myocardial injury and has significant cardioprotective potential.
},
 year = {2025}
}

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TY  - JOUR
T1  - Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid

AU  - Zhengyi Zhou
AU  - Zhuo Tang
AU  - Zihao Dai
AU  - Shuhe Xu
AU  - Yujie Ge
AU  - Yuemin Ding
AU  - Xiong Zhang
Y1  - 2025/11/22
PY  - 2025
N1  - https://doi.org/10.11648/j.bio.20251306.11
DO  - 10.11648/j.bio.20251306.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 106
EP  - 110
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20251306.11
AB  - This study comprehensively utilized toad hearts and myocardial cell models to explore the effects of acute lead exposure on myocardial contractile function and the therapeutic effect of alpha lipoic acid (ALA). The results showed that lead acetate (PbAc) dose dependently inhibited the contractile function of toad hearts. Acute treatment with 50, 150, and 450 mM PbAc reduced the amplitude of heart contraction to 92.8%, 78.0%, and 69.7% of the control before medication (PPPPP<0.05). This study indicates that PbAc can significantly inhibit myocardial contractility and reduce cell viability, while ALA can antagonize lead induced myocardial injury and has significant cardioprotective potential.

VL  - 13
IS  - 6
ER  -

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Author Information

Zhengyi Zhou

International Department, Hangzhou Wickham International School, Hangzhou, China
Zhuo Tang

International Department, Hangzhou Wickham International School, Hangzhou, China
Zihao Dai

International Department, Hangzhou Xizi Experimental School, Hangzhou, China
Shuhe Xu

International Department, Hangzhou Xizi Experimental School, Hangzhou, China
Yujie Ge

Department of Clinical Medicine, School of Medicine, Hangzhou City University, Hangzhou, China
Yuemin Ding

Department of Clinical Medicine, School of Medicine, Hangzhou City University, Hangzhou, China; Institute of Translational Medicine, Zhejiang University City College, Hangzhou, China; Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, Zhejiang University City College, Hangzhou, China

Contact Email

http://orcid.org/0000-0003-0547-5358
Xiong Zhang

Department of Basic Medical Science, Zhejiang University School of Medicine, Hangzhou, China

http://orcid.org/0000-0003-2235-569X

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Plain Text BibTeX RIS

APA Style

Zhou, Z., Tang, Z., Dai, Z., Xu, S., Ge, Y., et al. (2025). Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid. American Journal of Bioscience and Bioengineering, 13(6), 106-110. https://doi.org/10.11648/j.bio.20251306.11

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ACS Style

Zhou, Z.; Tang, Z.; Dai, Z.; Xu, S.; Ge, Y., et al. Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid. Am. J. BioSci. Bioeng. 2025, 13(6), 106-110. doi: 10.11648/j.bio.20251306.11

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AMA Style

Zhou Z, Tang Z, Dai Z, Xu S, Ge Y, et al. Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid. Am J BioSci Bioeng. 2025;13(6):106-110. doi: 10.11648/j.bio.20251306.11

Copy | Download

@article{10.11648/j.bio.20251306.11,
  author = {Zhengyi Zhou and Zhuo Tang and Zihao Dai and Shuhe Xu and Yujie Ge and Yuemin Ding and Xiong Zhang},
  title = {Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid
},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {13},
  number = {6},
  pages = {106-110},
  doi = {10.11648/j.bio.20251306.11},
  url = {https://doi.org/10.11648/j.bio.20251306.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20251306.11},
  abstract = {This study comprehensively utilized toad hearts and myocardial cell models to explore the effects of acute lead exposure on myocardial contractile function and the therapeutic effect of alpha lipoic acid (ALA). The results showed that lead acetate (PbAc) dose dependently inhibited the contractile function of toad hearts. Acute treatment with 50, 150, and 450 mM PbAc reduced the amplitude of heart contraction to 92.8%, 78.0%, and 69.7% of the control before medication (PPPPP<0.05). This study indicates that PbAc can significantly inhibit myocardial contractility and reduce cell viability, while ALA can antagonize lead induced myocardial injury and has significant cardioprotective potential.
},
 year = {2025}
}

Copy | Download

TY  - JOUR
T1  - Acute Lead Exposure Weakens Myocardial Contractile Function and the Therapeutic Effect of Alpha Lipoic Acid

AU  - Zhengyi Zhou
AU  - Zhuo Tang
AU  - Zihao Dai
AU  - Shuhe Xu
AU  - Yujie Ge
AU  - Yuemin Ding
AU  - Xiong Zhang
Y1  - 2025/11/22
PY  - 2025
N1  - https://doi.org/10.11648/j.bio.20251306.11
DO  - 10.11648/j.bio.20251306.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 106
EP  - 110
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20251306.11
AB  - This study comprehensively utilized toad hearts and myocardial cell models to explore the effects of acute lead exposure on myocardial contractile function and the therapeutic effect of alpha lipoic acid (ALA). The results showed that lead acetate (PbAc) dose dependently inhibited the contractile function of toad hearts. Acute treatment with 50, 150, and 450 mM PbAc reduced the amplitude of heart contraction to 92.8%, 78.0%, and 69.7% of the control before medication (PPPPP<0.05). This study indicates that PbAc can significantly inhibit myocardial contractility and reduce cell viability, while ALA can antagonize lead induced myocardial injury and has significant cardioprotective potential.

VL  - 13
IS  - 6
ER  -

Copy | Download