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Performance and Stability of Food Barley (Hordeum Vulgare L.) Varieties in the Highlands of East Hararghe, Ethiopia

Received: 12 June 2026     Accepted: 1 July 2026     Published: 30 July 2026
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Abstract

Food barley is a staple crop in the Ethiopian highlands, yet productivity remains low due to the use of low-yielding local varieties. This study evaluated the performance and adaptability of 11 food barley varieties (Abdane, Adoshe, Biftu, Guta, Robera, Cross#41/98, EH-1493, Harbu, HB-1965, HB-1966, and one local check) across six environments (Gurawa, Jarso, and Meta districts over 2019 and 2021 main cropping seasons). The experiment was conducted using a randomized complete block design with three replications. Combined analysis of variance revealed highly significant differences (p < 0.01) among varieties, environments, and their interactions for all traits. AMMI analysis indicated that environment explained 62.71% of total variation, genotype 5.74%, and genotype-by-environment interaction (GEI) 14.13%. The first two principal components of GEI accounted for 77.37% of the interaction variation. Varieties Abdane (4.81 t ha-1) and Robera (4.41 t ha-1) recorded the highest grain yields, outperforming the local check by 31.4% and 25.2%, respectively. GGE biplot analysis identified Abdane and Robera as the most stable and high-yielding varieties across environments, while Guta, Harbu, Cross#41/98, and the local check were unstable. Therefore, Abdane and Robera are recommended for further demonstration and production in East Hararghe highlands and similar agro-ecologies.

Published in American Journal of Bioscience and Bioengineering (Volume 14, Issue 4)
DOI 10.11648/j.bio.20261404.14
Page(s) 75-80
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2026. Published by Science Publishing Group

Keywords

Adaptability, AMMI, Food Barley, GGE Biplot, Grain Yield, Stability

1. Introduction
Barley (Hordeum vulgare L.) is a major cereal crop in Ethiopia, accounting for about 8% of total cereal production . It has been cultivated for centuries and is considered to have originated alongside plow agriculture . Barley is produced using various methods across diverse agroclimatic zones and ranks as the fourth most important cereal crop globally, behind maize, wheat, and rice .
Ethiopia ranks 21st globally in barley production, with barley being the fifth most significant cereal crop in terms of area and production, following maize, sorghum, tef, and wheat . In highland regions, barley is a staple cereal used to make injera, bread, soup, porridge, and both alcoholic and non-alcoholic beverages. Beyond grain value, barley straw is crucial for animal nutrition, especially during dry seasons when feed is scarce .
Barley is cultivated on approximately 0.93 million hectares in Ethiopia, with total production of 2.34 million tons. However, national average yield remains low at 2.52 t ha-1 . In the Oromia region, barley covers about 440,702 hectares with a yield of 2.80 t ha-1 . East Hararghe's food barley productivity is only 2.12 t ha-1, below the regional average .
Nearly 90% of barley grown by subsistence farmers consists of landraces , cultivated with minimal external inputs. East Hararghe zones face chronic food insecurity, with maize and sorghum as main food crops. Barley is poorly planted in many highland and midland areas due to farmers' reliance on low-yielding, disease-susceptible local varieties. Evaluating improved food barley varieties across different agro-ecologies can enhance yield potential. This study aimed to assess adaptability and performance of food barley varieties and identify high-yielding, disease-resistant, and stable varieties for East Hararghe highlands.
2. Materials and Methods
2.1. Experimental Sites and Genotypes
The experiment was conducted over two main cropping seasons (2019 and 2021) at three districts: Gurawa, Jarso, and Meta, resulting in six environments (Gurawa-19, Gurawa-21, Jarso-19, Jarso-21, Meta-19, Meta-21). Eleven food barley varieties were evaluated, including ten improved varieties and one local check (Table 1). Varieties were obtained from Holetta Agricultural Research Center (HARC) and Sinana Agricultural Research Center (SARC).
Table 1. Description of food barley varieties used in the study.

S. No.

Variety Name

Year of Release

Maintainer

1

Abdane

2011

Sinana ARC/OARI

2

Adoshe

2018

Sinana ARC/OARI

3

Biftu

2005

Sinana ARC/OARI

4

Cross#41/98

2012

Holeta ARC/EIAR

5

EH-1493

2012

Holeta ARC/EIAR

6

Guta

2007

Sinana ARC/OARI

7

Harbu

2004

Sinana ARC/OARI

8

HB-1965

2017

Holeta ARC/EIAR

9

HB-1966

2017

Holeta ARC/EIAR

10

Robera

2016

Sinana ARC/OARI

11

Local check

-

Farmers

2.2. Experimental Design and Crop Management
A randomized complete block design (RCBD) with three replications was used at each location. Each plot measured 1 m × 2.5 m, with six rows spaced 20 cm apart. Seeds were manually drilled at 125 kg ha-1. Fertilizer was applied at 100 kg ha-1 NPS (all at planting) and 100 kg ha-1 UREA (half at planting, half at tillering). Standard agronomic practices were followed.
2.3. Data Collection
Data were recorded for days to maturity (DTM), plant height (PH, cm), spike length (SL, cm), and grain yield (GY, t ha-1).
2.4. Statistical Analysis
Data were analyzed using R software. Combined ANOVA was performed with environments as random effects and genotypes as fixed effects. Bartlett's test confirmed homogeneity of variance. Treatment means were separated using Duncan's Multiple Range Test (DMRT) at p < 0.05. AMMI and GGE biplot analyses were conducted to partition GEI. The GGE biplot was constructed according to Yan et al. .
3. Results and Discussion
3.1. Combined Analysis of Variance
Combined ANOVA revealed highly significant differences (p < 0.01) among varieties for days to maturity, plant height, spike length, and grain yield (Table 2), consistent with previous reports . Environment significantly affected all traits, and genotype × environment interactions were also significant for all parameters.
Table 2. Combined ANOVA for yield and agronomic traits across six environments.

Source of Variation

Df

DTM

PLH

SPL

GYLD (Kg/ha)

Replication

2

7.84 ns

66.1ns

0.5921ns

3425858**

Genotypes

10

508.03***

553.4***

2.2637***

2931649***

Environment

5

2607.07***

7150.3***

21.2478***

64008222***

Genotypes X Environment

50

30.7***

193.5***

1.2953***

1442158***

Error

130

11.45

50

0.489

630969

** = p < 0.01; ns = non-significant. DTM = Days to maturity, PH = Plant height, SL = Spike length, GY = Grain yield.
3.2. Mean Performance of Varieties
Days to maturity ranged from 98.8 days (Harbu) to 115.9 days (Cross#41/98). Robera matured earliest (102.1 days), while Cross#41/98 and EH-1493 matured latest (Table 3). Plant height ranged from 85.4 cm (Adoshe) to 106.0 cm (Biftu). Spike length varied from 7.2 cm (Harbu, Robera) to 8.1 cm (Cross#41/98).
Table 3. Mean performance of food barley varieties across six environments.

Variety

DTM (days)

PLH (cm)

SPL (cm)

GYLD (Qt/ha)

Abdane

102.3 c

100.5 b

7.8 abc

4.81 a

Adoshe

106.7 b

85.4 e

7.6 bcd

3.98 bc

Biftu

103.3 c

106.0 a

7.3 cd

4.16 bc

Cross#41/98

115.9 a

102.1 ab

8.1 a

3.73 cd

EH1493

114 a

97.6 bc

8.0 ab

4.16 bc

Guta

102.4 c

99.7 b

7.6 bcd

3.62 cd

Harbu

98.8 d

100.1 b

7.2 d

3.78 cd

HB1965

102.3 c

92.0 d

8.0 ab

3.99 bc

HB1966

107.5 b

98.7 bc

7.3 cd

3.94 bc

Robera

102.1 c

93.9 cd

7.2 d

4.41 ab

Local

102.9 c

99.2 b

7.6 d

3.30 d

Mean

105.3

97.8

7.6

3.99

CV (%)

3.2

7.2

9.2

19.9

LSD (P< 5%)

2.2

4.7

0.5

0.52

Means followed by the same letter are not significantly different (p < 0.05).
The highest mean grain yield was recorded from Abdane (4.81 t ha-1) and Robera (4.41 t ha-1), while the local check yielded lowest (3.30 t ha-1). Across locations, mean grain yield ranged from 2.13 t ha-1 (Gurawa-21) to 5.44 t ha-1 (Meta-21) (Table 4). Abdane performed best at Gurawa-19, Gurawa-21, and Meta-19, while Robera performed best at Jarso-21.
Table 4. Mean grain yield (t ha-1) of varieties across six environments.

Variety

Jarso-19

Meta-19

Meta-21

Gurawa-19

Jarso-21

Gurawa-21

Mean

Abdane

5.89

53.97

5.81

5.00

3.10

3.64

4.81

Adoshe

5.77

30.00

5.11

4.36

3.62

2.02

3.98

Biftu

5.46

47.62

5.66

4.58

2.31

2.15

4.15

Cross#41/98

5.95

33.33

4.12

4.73

3.01

1.25

3.73

EH1493

5.68

39.37

6.17

4.45

2.73

1.99

4.16

Guta

4.80

43.97

5.33

4.11

1.56

1.54

3.62

Harbu

5.00

39.18

5.14

4.00

1.06

3.54

3.78

HB1965

6.53

30.48

6.20

4.29

2.72

1.14

3.99

HB1966

5.62

36.92

5.07

4.14

2.89

2.25

3.94

Robera

6.15

42.70

5.74

4.62

3.63

2.05

4.41

Local

2.74

43.02

5.44

3.66

1.76

1.89

3.30

E. Mean

5.42

40.05

5.44

4.36

2.58

2.13

3.99

3.3. AMMI Analysis for Grain Yield
AMMI analysis showed highly significant (p < 0.01) effects for environment, genotype, and GEI (Table 5). Environment captured the largest sum of squares (62.71%), followed by GEI (14.13%) and genotype (5.74%), indicating that environmental factors were the primary source of variation. This likely reflects rainfall distribution variability, soil type differences, and altitude range across locations .
Table 5. AMMI analysis of variance for grain yield across environments.

Source

d. f.

SS

MS

Explained%SS

Total

197

510.3

2.59

Treatments

65

421.5

6.48**

82.58

Genotypes

10

29.3

2.93**

5.74

Environments

5

320.

64.01**

62.71

Block

12

22.6

1.88**

Interactions

50

72.1

1.44**

14.13

IPCA 1

14

43.4

3.1**

60.18

IPCA 2

12

12.4

1.03*

17.19

IPCA3

10

9.5

0.95ns

13.19

Pooled Error

120

66.3

0.552

** = p < 0.01; * = p < 0.05; ns = non-significant.
The GEI was partitioned into IPCA1 (60.18%), IPCA2 (17.19%), and IPCA3 (13.19%), cumulatively explaining 90.56% of interaction. IPCA1 and IPCA2 were significant, justifying two-dimensional GGE biplot analysis .
3.4. GGE Biplot Analysis
Which-won-where pattern: The GGE biplot (Figure 1) showed PCA1 and PCA2 accounting for 46.8% and 26.97% of GGE variation, respectively (73.77% total). Vertex genotypes (farthest from origin) were Abdane, Robera, HB-1965, Cross#41/98, and Local check—either best or poorest performers. Abdane was the best genotype at Gurawa-21, Meta-19, and Gurawa-19. Cross#41/98, Adoshe, and HB-1965 performed best at Jarso-19 and Jarso-21.
Figure 1. Polygon view of GGE biplot showing which-won-where pattern of genotypes and environments.
Figure 2. Average environment coordination (AEC) view of GGE biplot for mean performance and stability.
Stability and mean performance: Using average environment coordination (AEC) (Figure 2), genotypes with high PC1 scores have high mean yield, while those with low PC2 scores are stable . Abdane, Robera, Biftu, and EH-1493 had above-average yields. Abdane showed the greatest stability (low PC2). The local check, Guta, Harbu, and Cross#41/98 were unstable.
Comparison with ideal genotype: An ideal genotype has high mean yield and high stability (close to concentric circle center). Abdane and Robera were closest to the ideal genotype (Figure 3), confirming their superior stability and yield.
Figure 3. GGE biplot comparing genotypes with ideal genotype for grain yield.
4. Conclusion and Recommendation
Significant variation among food barley varieties, environments, and their interactions was observed. Environment explained the largest portion (62.71%) of total variation, highlighting the importance of multi-environment testing. AMMI and GGE biplot analyses consistently identified Abdane and Robera as the highest-yielding and most stable varieties across all six environments. These varieties out yielded the local check by 31.4% and 25.2%, respectively. Varieties Abdane and Robera are recommended for further demonstration and popularization in East Hararghe highlands and similar agro-ecologies. Meta-21 and Gurawa-19 were the most favorable testing environments and should be prioritized in future breeding programs. The local check, Cross#41/98, Guta, and Harbu were unstable and not recommended for wider adoption.
Abbreviations

AEC

Average Environment Coordination

AMMI

Additive Main Effects and Multiplicative Interaction

ANOVA

Analysis of Variance

ARC

Agricultural Research Center

CV

Coefficient of Variation

Df

Degrees of Freedom

DMRT

Duncan's Multiple Range Test

DTM

Days to Maturity

EIAR

Ethiopian Institute of Agricultural Research

GEI

Genotype-by-Environment Interaction

GGE

Genotype and Genotype-by-Environment

GY

Grain Yield

HARC

Holetta Agricultural Research Center

IPCA

Interaction Principal Component Axis

LSD

Least Significant Difference

MS

Mean Square

ns

Non-significant

OARI

Oromia Agricultural Research Institute

PC1

Principal Component 1

PC2

Principal Component 2

PCA

Principal Component Analysis

PH

Plant Height

RCBD

Randomized Complete Block Design

SARC

Sinana Agricultural Research Center

SL

Spike Length

SS

Sum of Squares

Author Contributions
Zeleke Legesse: Conceptualization, Data curation, Formal Analysis, Investigation, Methodology, Resources, Software, Writing – original draft, Writing – review & editing
Jifara Gudeta: Data curation, Investigation, Methodology, Supervision, Writing – review & editing
Fikadu Tadesse: Data curation, Investigation, Methodology, Supervision
Birhanu Diribsa: Data curation, Investigation, Methodology, Supervision, Writing – review & editing
Conflicts of Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
References
[1] Wosene, G. A., Lakew, B., Haussmann, B. I. G., & Schmid, K. J. (2015). Ethiopian barley landraces show higher yield stability and comparable yield to improved varieties. Journal of Plant Breeding and Crop Science, 7(8), 275-291.
[2] Mulatu, B., & Grando, S. (Eds.). (2011). Barley Research and Development in Ethiopia. ICARDA.
[3] Megersa, G. (2014). Genetic erosion of barley in North Shewa Zone, Ethiopia. International Journal of Biodiversity and Conservation, 6(3), 280-289.
[4] FAO. (2017). Food and Agriculture Organization Statistical Yearbook. Rome.
[5] CSA. (2021). Agricultural Sample Survey Report on Area and Production of Major Crops. Addis Ababa, Ethiopia.
[6] Getachew, G., Tedila, A., Bediye, S., & Sebsibe, A. (1996). Improvement and utilization of barley straw. In Barley Research in Ethiopia (pp. 171-181). IAR/ICARDA.
[7] CSA. (2020). Agricultural Sample Survey Report. Addis Ababa, Ethiopia.
[8] Gebremedhin, W., Firew, M., & Tesfye, B. (2014). Stability analysis of food barley genotypes in Northern Ethiopia. African Crop Science Journal, 22(2), 145-153.
[9] Yan, W., Hunt, L. A., Sheng, Q., & Szlavnics, Z. (2000). Cultivar evaluation and mega-environment investigation based on the GGE biplot. Crop Science, 40, 597-605.
[10] Jimera, H., Hirpa, L., & Rao, C. P. (2015). Genetic variability in barley genotypes grown at Horo District, Western Ethiopia. Science, Technology and Arts Research Journal, 4(2), 1-9.
[11] Farshadfar, E., Safari, H., & Yaghotipoor, A. (2012). Chromosomal localization of QTLs controlling genotype × environment interaction in wheat. Journal of Agricultural Science, 4(12), 18.
[12] Yan, W., Kang, M. S., Ma, B., Woods, S., & Cornelius, P. L. (2007). GGE biplot vs. AMMI analysis of genotype-by-environment data. Crop Science, 47(2), 643-653.
[13] Purchase, J. L., Hatting, H., & van Deventer, C. S. (2000). Genotype by environment interaction of winter wheat in South Africa. South African Journal of Plant and Soil, 17, 101-107.
[14] Yan, W., & Tinker, N. A. (2006). Biplot analysis of multi-environment trial data. Canadian Journal of Plant Science, 86, 623-645.
Cite This Article
  • APA Style

    Legesse, Z., Gudeta, J., Tadesse, F., Diribsa, B. (2026). Performance and Stability of Food Barley (Hordeum Vulgare L.) Varieties in the Highlands of East Hararghe, Ethiopia. American Journal of Bioscience and Bioengineering, 14(4), 75-80. https://doi.org/10.11648/j.bio.20261404.14

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    ACS Style

    Legesse, Z.; Gudeta, J.; Tadesse, F.; Diribsa, B. Performance and Stability of Food Barley (Hordeum Vulgare L.) Varieties in the Highlands of East Hararghe, Ethiopia. Am. J. BioSci. Bioeng. 2026, 14(4), 75-80. doi: 10.11648/j.bio.20261404.14

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    AMA Style

    Legesse Z, Gudeta J, Tadesse F, Diribsa B. Performance and Stability of Food Barley (Hordeum Vulgare L.) Varieties in the Highlands of East Hararghe, Ethiopia. Am J BioSci Bioeng. 2026;14(4):75-80. doi: 10.11648/j.bio.20261404.14

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  • @article{10.11648/j.bio.20261404.14,
      author = {Zeleke Legesse and Jifara Gudeta and Fikadu Tadesse and Birhanu Diribsa},
      title = {Performance and Stability of Food Barley (Hordeum Vulgare L.) Varieties in the Highlands of East Hararghe, Ethiopia},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {14},
      number = {4},
      pages = {75-80},
      doi = {10.11648/j.bio.20261404.14},
      url = {https://doi.org/10.11648/j.bio.20261404.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20261404.14},
      abstract = {Food barley is a staple crop in the Ethiopian highlands, yet productivity remains low due to the use of low-yielding local varieties. This study evaluated the performance and adaptability of 11 food barley varieties (Abdane, Adoshe, Biftu, Guta, Robera, Cross#41/98, EH-1493, Harbu, HB-1965, HB-1966, and one local check) across six environments (Gurawa, Jarso, and Meta districts over 2019 and 2021 main cropping seasons). The experiment was conducted using a randomized complete block design with three replications. Combined analysis of variance revealed highly significant differences (p -1) and Robera (4.41 t ha-1) recorded the highest grain yields, outperforming the local check by 31.4% and 25.2%, respectively. GGE biplot analysis identified Abdane and Robera as the most stable and high-yielding varieties across environments, while Guta, Harbu, Cross#41/98, and the local check were unstable. Therefore, Abdane and Robera are recommended for further demonstration and production in East Hararghe highlands and similar agro-ecologies.},
     year = {2026}
    }
    

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  • TY  - JOUR
    T1  - Performance and Stability of Food Barley (Hordeum Vulgare L.) Varieties in the Highlands of East Hararghe, Ethiopia
    AU  - Zeleke Legesse
    AU  - Jifara Gudeta
    AU  - Fikadu Tadesse
    AU  - Birhanu Diribsa
    Y1  - 2026/07/30
    PY  - 2026
    N1  - https://doi.org/10.11648/j.bio.20261404.14
    DO  - 10.11648/j.bio.20261404.14
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 75
    EP  - 80
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20261404.14
    AB  - Food barley is a staple crop in the Ethiopian highlands, yet productivity remains low due to the use of low-yielding local varieties. This study evaluated the performance and adaptability of 11 food barley varieties (Abdane, Adoshe, Biftu, Guta, Robera, Cross#41/98, EH-1493, Harbu, HB-1965, HB-1966, and one local check) across six environments (Gurawa, Jarso, and Meta districts over 2019 and 2021 main cropping seasons). The experiment was conducted using a randomized complete block design with three replications. Combined analysis of variance revealed highly significant differences (p -1) and Robera (4.41 t ha-1) recorded the highest grain yields, outperforming the local check by 31.4% and 25.2%, respectively. GGE biplot analysis identified Abdane and Robera as the most stable and high-yielding varieties across environments, while Guta, Harbu, Cross#41/98, and the local check were unstable. Therefore, Abdane and Robera are recommended for further demonstration and production in East Hararghe highlands and similar agro-ecologies.
    VL  - 14
    IS  - 4
    ER  - 

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Author Information
  • Department of Cereal, Fadis Agricultural Research Center of Oromia Agricultural Research Institute (OARI), Harar, Ethiopia

  • Department of Cereal, Fadis Agricultural Research Center of Oromia Agricultural Research Institute (OARI), Harar, Ethiopia

  • Department of Cereal, Fadis Agricultural Research Center of Oromia Agricultural Research Institute (OARI), Harar, Ethiopia

  • Department of Cereal, Fadis Agricultural Research Center of Oromia Agricultural Research Institute (OARI), Harar, Ethiopia