A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang

Hongxia Wu

doi:doi:10.11648/j.cmr.20140305.16

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A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang

Hongxia Wu

Published in Clinical Medicine Research (Volume 3, Issue 5)

Received: 11 August 2014 Accepted: 5 September 2014 Published: 20 September 2014

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Abstract

The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present report has revealed that the sequence of a new gene, aadA5, combining with the trimethoprim resistance gene dfr17 occured in a class 1integron. The integron was identified in a nosocomial pathogen Enterobacter cloacae isolates, which indicated that integrons are specialized genetic elements that were capable of capturing, integrating and mobilizing gene-cassette by site-specific recombination and integron was a dangerous factor to lead to bacterial resistance.

Published in	Clinical Medicine Research (Volume 3, Issue 5)
DOI	10.11648/j.cmr.20140305.16
Page(s)	142-144
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

E. Cloacae, Class 1 Integron, Resistance

References

[1]	Jeong SH, Lee K, Chong Y, Yum JH, Lee SH, Choi HJ, Kim JM, Park KH, Han BH, Lee SW, Jeong TS,. Characterization of a new integron containing VIM-2, a metallo- beta-lactamase gene cassette, in a clinical isolate of Enterobacter cloacae. J Antimicrob Chemother. 2003;51(2):397-400.
[2]	Ahmed AM, Nakano H, Shimamoto T, Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron. J Antimicrob Chemother. 2005, 55(3):371-4.
[3]	Hall RM, Mobile gene cassettes and integrons: moving antibiotic resistance genes in gram-negative bacteria. Ciba Found Symp. 1997, 207:192–202; discussion 202–5.
[4]	Hollingshead S, Vapnek D, Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenyltransferase. Plasmid. 1985,13:17–30.
[5]	Clark NC, Olsvik O, Swenson JM, Spiegel CA, Tenover FC, Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis. Antimicrob. Agents Chemother. 1999, 43:157–160.
[6]	National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically: Approved Standard. 1997. M7-A4, M7-A4. NCCLS, Villanova, PA, USA.
[7]	Lévesque C, Piché L, Larose C, Roy PH, PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob. Agents Chemother. 1995, 39, 185–191.
[8]	Valverde AR, Canto´n JC, Galán JC, In117, an unusual In0-like class 1 integron containing CR1 and blaCTX-M-2 and associated with a Tn21-like element. Antimicrob. Agents Chemother. 2005, 50:799–802.
[9]	Lee K, Lim JB, Yum JH, Yong D, Chong Y, Kim JM, Livermore DM, bla(VIM-2) cassette-containing novel integrons in metallo-beta-lactamase-producing Pseudomonas aeruginosa and Pseudomonas putida isolates disseminated in a Korean hospital. Antimicrob. Agents Chemother. 2002, 46, 1053–8.
[10]	Hall RM, Collis CM, Kim MJ, Partridge SR, Recchia GD, Stokes HW, Mobile gene cassettes and integrons in evolution. Ann N Y Acad Sci. 1999, 870: 68–80.
[11]	Sun C, Su Z, Zhou C, Liu Y, Yuan H, Yin J, Xu H, Complex class 1 integron containing bla ( CTX-M-1 ) genes isolated from Escherichia coli: a potentially novel resistant gene-capturing tool kit.Curr Microbiol. 2012,64(3):265-70.
[12]	Xu H, Su Z, Wang S, Dai X, Chen J, Kong F, Li Y, Peng S, Shao Q, Lu L, Ezaki T, Four novel resistance integron gene-cassette occurrences in bacterial isolates from zhenjiang, china. Curr Microbiol. 2009, 59(2):113-7.

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APA Style

Hongxia Wu. (2014). A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang. Clinical Medicine Research, 3(5), 142-144. https://doi.org/10.11648/j.cmr.20140305.16

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ACS Style

Hongxia Wu. A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang. Clin. Med. Res. 2014, 3(5), 142-144. doi: 10.11648/j.cmr.20140305.16

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AMA Style

Hongxia Wu. A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang. Clin Med Res. 2014;3(5):142-144. doi: 10.11648/j.cmr.20140305.16

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@article{10.11648/j.cmr.20140305.16,
  author = {Hongxia Wu},
  title = {A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang},
  journal = {Clinical Medicine Research},
  volume = {3},
  number = {5},
  pages = {142-144},
  doi = {10.11648/j.cmr.20140305.16},
  url = {https://doi.org/10.11648/j.cmr.20140305.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20140305.16},
  abstract = {The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present report has revealed that the sequence of a new gene, aadA5, combining with the trimethoprim resistance gene dfr17 occured in a class 1integron. The integron was identified in a nosocomial pathogen Enterobacter cloacae isolates, which indicated that integrons are specialized genetic elements that were capable of capturing, integrating and mobilizing gene-cassette by site-specific recombination and integron was a dangerous factor to lead to bacterial resistance.},
 year = {2014}
}

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TY  - JOUR
T1  - A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang
AU  - Hongxia Wu
Y1  - 2014/09/20
PY  - 2014
N1  - https://doi.org/10.11648/j.cmr.20140305.16
DO  - 10.11648/j.cmr.20140305.16
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 142
EP  - 144
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20140305.16
AB  - The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present report has revealed that the sequence of a new gene, aadA5, combining with the trimethoprim resistance gene dfr17 occured in a class 1integron. The integron was identified in a nosocomial pathogen Enterobacter cloacae isolates, which indicated that integrons are specialized genetic elements that were capable of capturing, integrating and mobilizing gene-cassette by site-specific recombination and integron was a dangerous factor to lead to bacterial resistance.
VL  - 3
IS  - 5
ER  -

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Author Information

Hongxia Wu

Department of Microbiology, Jiangyin people’s hospital, Jiangyin, China, 214400

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APA Style

Hongxia Wu. (2014). A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang. Clinical Medicine Research, 3(5), 142-144. https://doi.org/10.11648/j.cmr.20140305.16

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ACS Style

Hongxia Wu. A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang. Clin. Med. Res. 2014, 3(5), 142-144. doi: 10.11648/j.cmr.20140305.16

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AMA Style

Hongxia Wu. A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang. Clin Med Res. 2014;3(5):142-144. doi: 10.11648/j.cmr.20140305.16

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@article{10.11648/j.cmr.20140305.16,
  author = {Hongxia Wu},
  title = {A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang},
  journal = {Clinical Medicine Research},
  volume = {3},
  number = {5},
  pages = {142-144},
  doi = {10.11648/j.cmr.20140305.16},
  url = {https://doi.org/10.11648/j.cmr.20140305.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20140305.16},
  abstract = {The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present report has revealed that the sequence of a new gene, aadA5, combining with the trimethoprim resistance gene dfr17 occured in a class 1integron. The integron was identified in a nosocomial pathogen Enterobacter cloacae isolates, which indicated that integrons are specialized genetic elements that were capable of capturing, integrating and mobilizing gene-cassette by site-specific recombination and integron was a dangerous factor to lead to bacterial resistance.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - A Novel Streptomycin and Spectinomycin Resistance Gene Cassette Occurence in E. cloacae isolated from Zhenjiang
AU  - Hongxia Wu
Y1  - 2014/09/20
PY  - 2014
N1  - https://doi.org/10.11648/j.cmr.20140305.16
DO  - 10.11648/j.cmr.20140305.16
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 142
EP  - 144
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20140305.16
AB  - The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present report has revealed that the sequence of a new gene, aadA5, combining with the trimethoprim resistance gene dfr17 occured in a class 1integron. The integron was identified in a nosocomial pathogen Enterobacter cloacae isolates, which indicated that integrons are specialized genetic elements that were capable of capturing, integrating and mobilizing gene-cassette by site-specific recombination and integron was a dangerous factor to lead to bacterial resistance.
VL  - 3
IS  - 5
ER  -

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