,
The rising antibiotic resistance of UPEC strains highlights the need for alternate preventive measures for urinary tract infections (UTIs) brought on by Uropathogenic Escherichia coli (UPEC) strain Uti89. This in silico study (2022-25) used a subtractive genomics-based reverse vaccinology approach; data extraction, protein analysis, epitope prediction, docking analysis, protein-ligand complexes, molecular dynamics, Human immune simulation, fragment Inserted in vector (pET-28a (+) were all steps in the study's multi-step methodology to find a potential vaccine candidate against UTIs. 3,840 non-homologous proteins unique to UPEC-UTI89 were found by extracting data from databases. The pathogen's essential genes were identified, and predict on the bases of subcellular localization assisted in choosing among membrane-related proteins that would make good vaccine targets. Seventy-one (71) proteins were identified through antigenic analysis as potential vaccine candidates. 7 outer membrane proteins with potential vaccine properties were found through screening and shortlisted for vaccine construction. Investigation of the metabolic pathways in UPEC-UTI89 revealed both typical and unusual pathways. Several tools were used to predict epitopes for B-cell and T-cell immune responses. MHC-I and MHC-II cluster analyses were carried out to confirm the prediction of potential epitopes. The resulting epitopes demonstrated potential for eliciting a powerful immune reaction against UTIs brought on by UPEC UTI89. By identifying potential vaccine targets and epitopes using subtractive genomics and reverse vaccination approach, this in silico study aids ongoing efforts to develop an effective UTI vaccine. The resulting epitopes demonstrated potential for eliciting a powerful immune reaction against UTIs brought on by UPEC UTI89.
| Published in | International Journal of Immunology (Volume 14, Issue 2) |
| DOI | 10.11648/j.iji.20261402.11 |
| Page(s) | 38-56 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2026. Published by Science Publishing Group |
Subtractive Genomic Analysis, Reverse Vaccinology Approach, Epitope Prediction, Vaccine Construct, UPEC-UTI89
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APA Style
Zaib, G., Javed, S. (2026). In-silico Vaccine Candidate Peptides Against Urinary Tract Infection in Humans Using Uropathogenic Escherichia Coli-Uti89 Strain. International Journal of Immunology, 14(2), 38-56. https://doi.org/10.11648/j.iji.20261402.11
ACS Style
Zaib, G.; Javed, S. In-silico Vaccine Candidate Peptides Against Urinary Tract Infection in Humans Using Uropathogenic Escherichia Coli-Uti89 Strain. Int. J. Immunol. 2026, 14(2), 38-56. doi: 10.11648/j.iji.20261402.11
AMA Style
Zaib G, Javed S. In-silico Vaccine Candidate Peptides Against Urinary Tract Infection in Humans Using Uropathogenic Escherichia Coli-Uti89 Strain. Int J Immunol. 2026;14(2):38-56. doi: 10.11648/j.iji.20261402.11
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Download@article{10.11648/j.iji.20261402.11,
author = {Gul Zaib and Saira Javed},
title = {In-silico Vaccine Candidate Peptides Against Urinary Tract Infection in Humans Using Uropathogenic Escherichia
Coli-Uti89 Strain},
journal = {International Journal of Immunology},
volume = {14},
number = {2},
pages = {38-56},
doi = {10.11648/j.iji.20261402.11},
url = {https://doi.org/10.11648/j.iji.20261402.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20261402.11},
abstract = {The rising antibiotic resistance of UPEC strains highlights the need for alternate preventive measures for urinary tract infections (UTIs) brought on by Uropathogenic Escherichia coli (UPEC) strain Uti89. This in silico study (2022-25) used a subtractive genomics-based reverse vaccinology approach; data extraction, protein analysis, epitope prediction, docking analysis, protein-ligand complexes, molecular dynamics, Human immune simulation, fragment Inserted in vector (pET-28a (+) were all steps in the study's multi-step methodology to find a potential vaccine candidate against UTIs. 3,840 non-homologous proteins unique to UPEC-UTI89 were found by extracting data from databases. The pathogen's essential genes were identified, and predict on the bases of subcellular localization assisted in choosing among membrane-related proteins that would make good vaccine targets. Seventy-one (71) proteins were identified through antigenic analysis as potential vaccine candidates. 7 outer membrane proteins with potential vaccine properties were found through screening and shortlisted for vaccine construction. Investigation of the metabolic pathways in UPEC-UTI89 revealed both typical and unusual pathways. Several tools were used to predict epitopes for B-cell and T-cell immune responses. MHC-I and MHC-II cluster analyses were carried out to confirm the prediction of potential epitopes. The resulting epitopes demonstrated potential for eliciting a powerful immune reaction against UTIs brought on by UPEC UTI89. By identifying potential vaccine targets and epitopes using subtractive genomics and reverse vaccination approach, this in silico study aids ongoing efforts to develop an effective UTI vaccine. The resulting epitopes demonstrated potential for eliciting a powerful immune reaction against UTIs brought on by UPEC UTI89.},
year = {2026}
}
TY - JOUR T1 - In-silico Vaccine Candidate Peptides Against Urinary Tract Infection in Humans Using Uropathogenic Escherichia Coli-Uti89 Strain AU - Gul Zaib AU - Saira Javed Y1 - 2026/09/28 PY - 2026 N1 - https://doi.org/10.11648/j.iji.20261402.11 DO - 10.11648/j.iji.20261402.11 T2 - International Journal of Immunology JF - International Journal of Immunology JO - International Journal of Immunology SP - 38 EP - 56 PB - Science Publishing Group SN - 2329-1753 UR - https://doi.org/10.11648/j.iji.20261402.11 AB - The rising antibiotic resistance of UPEC strains highlights the need for alternate preventive measures for urinary tract infections (UTIs) brought on by Uropathogenic Escherichia coli (UPEC) strain Uti89. This in silico study (2022-25) used a subtractive genomics-based reverse vaccinology approach; data extraction, protein analysis, epitope prediction, docking analysis, protein-ligand complexes, molecular dynamics, Human immune simulation, fragment Inserted in vector (pET-28a (+) were all steps in the study's multi-step methodology to find a potential vaccine candidate against UTIs. 3,840 non-homologous proteins unique to UPEC-UTI89 were found by extracting data from databases. The pathogen's essential genes were identified, and predict on the bases of subcellular localization assisted in choosing among membrane-related proteins that would make good vaccine targets. Seventy-one (71) proteins were identified through antigenic analysis as potential vaccine candidates. 7 outer membrane proteins with potential vaccine properties were found through screening and shortlisted for vaccine construction. Investigation of the metabolic pathways in UPEC-UTI89 revealed both typical and unusual pathways. Several tools were used to predict epitopes for B-cell and T-cell immune responses. MHC-I and MHC-II cluster analyses were carried out to confirm the prediction of potential epitopes. The resulting epitopes demonstrated potential for eliciting a powerful immune reaction against UTIs brought on by UPEC UTI89. By identifying potential vaccine targets and epitopes using subtractive genomics and reverse vaccination approach, this in silico study aids ongoing efforts to develop an effective UTI vaccine. The resulting epitopes demonstrated potential for eliciting a powerful immune reaction against UTIs brought on by UPEC UTI89. VL - 14 IS - 2 ER -
Department of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan
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