<i>In Vitro</i> Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (<i>Jatropha curcas </i>L.)

Md. Humayun Kabir; Md. Monirul Islam; Pronabananda Das; Mazedul Anwar Razib; Ahmad Nazri Karim Mamun

doi:doi:10.11648/j.plant.20251304.13

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In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.)

Md. Humayun Kabir

, Md. Monirul Islam^*

, Pronabananda Das

, Mazedul Anwar Razib

, Ahmad Nazri Karim Mamun

Published in Plant (Volume 13, Issue 4)

Received: 5 November 2025 Accepted: 18 November 2025 Published: 24 December 2025

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Abstract

In vitro regeneration of Jatropha curcus L. was established from different explants such as shoot tips, cotyledons and nodal segments using different concentrations and combinations of plant growth regulators and additives. The nodal segments were found superior explants to shoot tips and cotyledons. The highest result obtained on multiple shoot formation from nodal explants using 2.0 mg/l BA + 2.0 mg/l kin. in MS supplemented medium, in which 70% of explants responded for multiple shooting after 30 days of inoculation period. The average number of shoots and leaves/explants were 4.2  0.64 and 2.90  0.42 in this medium 90 days after inoculation respectively. The average shoot length of 3.62  0.29 cm was also found in this same media and condition. Regenerated shoots were rooted well when transferred to of half strength of MS + 2.5 mg/l lBA, in which 50% of shoots responded after 30 days of inoculation period. The average number of roots/in-vitro raised shoots of 3.2  0.65 and the average root length of 3.5 0.29 cm were recorded in this medium after 60 days of inoculation. Plantlet regeneration was observed with low frequency in shoot tip and cotyledon explants. Most of the regenerants initially produced a clump of meristems and tended to form calli rather than multiple shoot formations.

Published in	Plant (Volume 13, Issue 4)
DOI	10.11648/j.plant.20251304.13
Page(s)	191-197
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

Jatropha, Shoot Tip, Cotyledon, Nodal Fraction, Regeneration

References

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APA Style

Kabir, M. H., Islam, M. M., Das, P., Razib, M. A., Mamun, A. N. K. (2025). In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.). Plant, 13(4), 191-197. https://doi.org/10.11648/j.plant.20251304.13

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ACS Style

Kabir, M. H.; Islam, M. M.; Das, P.; Razib, M. A.; Mamun, A. N. K. In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.). Plant. 2025, 13(4), 191-197. doi: 10.11648/j.plant.20251304.13

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AMA Style

Kabir MH, Islam MM, Das P, Razib MA, Mamun ANK. In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.). Plant. 2025;13(4):191-197. doi: 10.11648/j.plant.20251304.13

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@article{10.11648/j.plant.20251304.13,
  author = {Md. Humayun Kabir and Md. Monirul Islam and Pronabananda Das and Mazedul Anwar Razib and Ahmad Nazri Karim Mamun},
  title = {In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.)},
  journal = {Plant},
  volume = {13},
  number = {4},
  pages = {191-197},
  doi = {10.11648/j.plant.20251304.13},
  url = {https://doi.org/10.11648/j.plant.20251304.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20251304.13},
  abstract = {In vitro regeneration of Jatropha curcus L. was established from different explants such as shoot tips, cotyledons and nodal segments using different concentrations and combinations of plant growth regulators and additives. The nodal segments were found superior explants to shoot tips and cotyledons. The highest result obtained on multiple shoot formation from nodal explants using 2.0 mg/l BA + 2.0 mg/l kin. in MS supplemented medium, in which 70% of explants responded for multiple shooting after 30 days of inoculation period. The average number of shoots and leaves/explants were 4.2  0.64 and 2.90  0.42 in this medium 90 days after inoculation respectively. The average shoot length of 3.62  0.29 cm was also found in this same media and condition. Regenerated shoots were rooted well when transferred to of half strength of MS + 2.5 mg/l lBA, in which 50% of shoots responded after 30 days of inoculation period. The average number of roots/in-vitro raised shoots of 3.2  0.65 and the average root length of 3.5 0.29 cm were recorded in this medium after 60 days of inoculation. Plantlet regeneration was observed with low frequency in shoot tip and cotyledon explants. Most of the regenerants initially produced a clump of meristems and tended to form calli rather than multiple shoot formations.},
 year = {2025}
}

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TY - JOUR
T1 - In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.)
AU - Md. Humayun Kabir
AU - Md. Monirul Islam
AU - Pronabananda Das
AU - Mazedul Anwar Razib
AU - Ahmad Nazri Karim Mamun
Y1 - 2025/12/24
PY - 2025
N1 - https://doi.org/10.11648/j.plant.20251304.13
DO - 10.11648/j.plant.20251304.13
T2 - Plant
JF - Plant
JO - Plant
SP - 191
EP - 197
PB - Science Publishing Group
SN - 2331-0677
UR - https://doi.org/10.11648/j.plant.20251304.13
AB - In vitro regeneration of Jatropha curcus L. was established from different explants such as shoot tips, cotyledons and nodal segments using different concentrations and combinations of plant growth regulators and additives. The nodal segments were found superior explants to shoot tips and cotyledons. The highest result obtained on multiple shoot formation from nodal explants using 2.0 mg/l BA + 2.0 mg/l kin. in MS supplemented medium, in which 70% of explants responded for multiple shooting after 30 days of inoculation period. The average number of shoots and leaves/explants were 4.2  0.64 and 2.90  0.42 in this medium 90 days after inoculation respectively. The average shoot length of 3.62  0.29 cm was also found in this same media and condition. Regenerated shoots were rooted well when transferred to of half strength of MS + 2.5 mg/l lBA, in which 50% of shoots responded after 30 days of inoculation period. The average number of roots/in-vitro raised shoots of 3.2  0.65 and the average root length of 3.5 0.29 cm were recorded in this medium after 60 days of inoculation. Plantlet regeneration was observed with low frequency in shoot tip and cotyledon explants. Most of the regenerants initially produced a clump of meristems and tended to form calli rather than multiple shoot formations.
VL - 13
IS - 4
ER -

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Author Information

Md. Humayun Kabir

Plant Biotechnology and Genetic Engineering Division, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh

Contact Email

http://orcid.org/0009-0009-8826-7386
Md. Monirul Islam

Plant Biotechnology and Genetic Engineering Division, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh

Contact Email

http://orcid.org/0000-0002-4719-1938
Pronabananda Das

Plant Biotechnology and Genetic Engineering Division, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh

Contact Email

http://orcid.org/0000-0002-0376-7106
Mazedul Anwar Razib

Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh

Contact Email

http://orcid.org/0009-0005-4942-7616
Ahmad Nazri Karim Mamun

Plant Biotechnology and Genetic Engineering Division, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh

Contact Email

http://orcid.org/0000-0002-2383-4206

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APA Style

Kabir, M. H., Islam, M. M., Das, P., Razib, M. A., Mamun, A. N. K. (2025). In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.). Plant, 13(4), 191-197. https://doi.org/10.11648/j.plant.20251304.13

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Kabir, M. H.; Islam, M. M.; Das, P.; Razib, M. A.; Mamun, A. N. K. In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.). Plant. 2025, 13(4), 191-197. doi: 10.11648/j.plant.20251304.13

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AMA Style

Kabir MH, Islam MM, Das P, Razib MA, Mamun ANK. In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.). Plant. 2025;13(4):191-197. doi: 10.11648/j.plant.20251304.13

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@article{10.11648/j.plant.20251304.13,
  author = {Md. Humayun Kabir and Md. Monirul Islam and Pronabananda Das and Mazedul Anwar Razib and Ahmad Nazri Karim Mamun},
  title = {In Vitro Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (Jatropha curcas L.)},
  journal = {Plant},
  volume = {13},
  number = {4},
  pages = {191-197},
  doi = {10.11648/j.plant.20251304.13},
  url = {https://doi.org/10.11648/j.plant.20251304.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20251304.13},
  abstract = {In vitro regeneration of Jatropha curcus L. was established from different explants such as shoot tips, cotyledons and nodal segments using different concentrations and combinations of plant growth regulators and additives. The nodal segments were found superior explants to shoot tips and cotyledons. The highest result obtained on multiple shoot formation from nodal explants using 2.0 mg/l BA + 2.0 mg/l kin. in MS supplemented medium, in which 70% of explants responded for multiple shooting after 30 days of inoculation period. The average number of shoots and leaves/explants were 4.2  0.64 and 2.90  0.42 in this medium 90 days after inoculation respectively. The average shoot length of 3.62  0.29 cm was also found in this same media and condition. Regenerated shoots were rooted well when transferred to of half strength of MS + 2.5 mg/l lBA, in which 50% of shoots responded after 30 days of inoculation period. The average number of roots/in-vitro raised shoots of 3.2  0.65 and the average root length of 3.5 0.29 cm were recorded in this medium after 60 days of inoculation. Plantlet regeneration was observed with low frequency in shoot tip and cotyledon explants. Most of the regenerants initially produced a clump of meristems and tended to form calli rather than multiple shoot formations.},
 year = {2025}
}

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