,
Aniefiok Livinus,
Akpabio Udoh Julius,
Nsikak Andrew Abraham,
Christina Ime Udosen
Microbial degradation of paraffinic compounds has gained considerable interest due to their easy handling, eco-friendliness, high operational safety for sustainable development, low toxicity, non-carcinogenic nature, non-combustibility, and widespread presence of microorganisms. Biosurfactants increase bioavailability and help enhance contact between pollutants and microorganisms, thereby facilitating uptake and degradation, as well as the amelioration (remediation) of hydrocarbon-polluted environments. It also acts as a waxy depressant, enhancing the flow of crude during production. This study investigated the biosurfactant-producing potential of autochthonous bacterial species associated with waxy crude oil. The culturable autochthonous heterotrophic bacterial density in the waxy samples ranged from 1.3 ± 0.3 x 104 to 2.6 ± 0.3 x 104 CFU/ml. Four distinct bacterial species were isolated, characterized, and identified as Bacillus cereus, B. subtilis, Pseudomonas aeruginosa, and Micrococcus sp. Analysis of the biosurfactant potentials of the isolates cell-free supernatants revealed varying biosurfactant potentials, with Pseudomonas aeruginosa culture extract having the best oil spread result and biosurfactant potential (% EC24 = 52.05), followed by B. subtilis (% EC24 = 50.0), then Micrococcus sp. (% EC24 = 48.7) and B. cereus (% EC24 = 47.5). All the isolates' cell-free extracts exhibited β-hemolysis. The potential of these autochthonous bacterial communities would be explored in the future as depressants for waxy crude oil treatment and for in the oil and gas sector, microbial enhanced oil recovery.
| Published in | Petroleum Science and Engineering (Volume 9, Issue 2) |
| DOI | 10.11648/j.pse.20250902.19 |
| Page(s) | 134-140 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Biosurfactant Production, Bacteria, Waxy Crude, Wax Treatment
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APA Style
Friday, F. S., Livinus, A., Julius, A. U., Abraham, N. A., Udosen, C. I. (2025). Biosurfactant Production Potentials of Autochthonous Bacterial Species Associated with Waxy Crude Oil. Petroleum Science and Engineering, 9(2), 134-140. https://doi.org/10.11648/j.pse.20250902.19
ACS Style
Friday, F. S.; Livinus, A.; Julius, A. U.; Abraham, N. A.; Udosen, C. I. Biosurfactant Production Potentials of Autochthonous Bacterial Species Associated with Waxy Crude Oil. Pet. Sci. Eng. 2025, 9(2), 134-140. doi: 10.11648/j.pse.20250902.19
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Download@article{10.11648/j.pse.20250902.19,
author = {Festus Sunday Friday and Aniefiok Livinus and Akpabio Udoh Julius and Nsikak Andrew Abraham and Christina Ime Udosen},
title = {Biosurfactant Production Potentials of Autochthonous Bacterial Species Associated with Waxy Crude Oil},
journal = {Petroleum Science and Engineering},
volume = {9},
number = {2},
pages = {134-140},
doi = {10.11648/j.pse.20250902.19},
url = {https://doi.org/10.11648/j.pse.20250902.19},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.pse.20250902.19},
abstract = {Microbial degradation of paraffinic compounds has gained considerable interest due to their easy handling, eco-friendliness, high operational safety for sustainable development, low toxicity, non-carcinogenic nature, non-combustibility, and widespread presence of microorganisms. Biosurfactants increase bioavailability and help enhance contact between pollutants and microorganisms, thereby facilitating uptake and degradation, as well as the amelioration (remediation) of hydrocarbon-polluted environments. It also acts as a waxy depressant, enhancing the flow of crude during production. This study investigated the biosurfactant-producing potential of autochthonous bacterial species associated with waxy crude oil. The culturable autochthonous heterotrophic bacterial density in the waxy samples ranged from 1.3 ± 0.3 x 104 to 2.6 ± 0.3 x 104 CFU/ml. Four distinct bacterial species were isolated, characterized, and identified as Bacillus cereus, B. subtilis, Pseudomonas aeruginosa, and Micrococcus sp. Analysis of the biosurfactant potentials of the isolates cell-free supernatants revealed varying biosurfactant potentials, with Pseudomonas aeruginosa culture extract having the best oil spread result and biosurfactant potential (% EC24 = 52.05), followed by B. subtilis (% EC24 = 50.0), then Micrococcus sp. (% EC24 = 48.7) and B. cereus (% EC24 = 47.5). All the isolates' cell-free extracts exhibited β-hemolysis. The potential of these autochthonous bacterial communities would be explored in the future as depressants for waxy crude oil treatment and for in the oil and gas sector, microbial enhanced oil recovery.},
year = {2025}
}
TY - JOUR T1 - Biosurfactant Production Potentials of Autochthonous Bacterial Species Associated with Waxy Crude Oil AU - Festus Sunday Friday AU - Aniefiok Livinus AU - Akpabio Udoh Julius AU - Nsikak Andrew Abraham AU - Christina Ime Udosen Y1 - 2025/12/19 PY - 2025 N1 - https://doi.org/10.11648/j.pse.20250902.19 DO - 10.11648/j.pse.20250902.19 T2 - Petroleum Science and Engineering JF - Petroleum Science and Engineering JO - Petroleum Science and Engineering SP - 134 EP - 140 PB - Science Publishing Group SN - 2640-4516 UR - https://doi.org/10.11648/j.pse.20250902.19 AB - Microbial degradation of paraffinic compounds has gained considerable interest due to their easy handling, eco-friendliness, high operational safety for sustainable development, low toxicity, non-carcinogenic nature, non-combustibility, and widespread presence of microorganisms. Biosurfactants increase bioavailability and help enhance contact between pollutants and microorganisms, thereby facilitating uptake and degradation, as well as the amelioration (remediation) of hydrocarbon-polluted environments. It also acts as a waxy depressant, enhancing the flow of crude during production. This study investigated the biosurfactant-producing potential of autochthonous bacterial species associated with waxy crude oil. The culturable autochthonous heterotrophic bacterial density in the waxy samples ranged from 1.3 ± 0.3 x 104 to 2.6 ± 0.3 x 104 CFU/ml. Four distinct bacterial species were isolated, characterized, and identified as Bacillus cereus, B. subtilis, Pseudomonas aeruginosa, and Micrococcus sp. Analysis of the biosurfactant potentials of the isolates cell-free supernatants revealed varying biosurfactant potentials, with Pseudomonas aeruginosa culture extract having the best oil spread result and biosurfactant potential (% EC24 = 52.05), followed by B. subtilis (% EC24 = 50.0), then Micrococcus sp. (% EC24 = 48.7) and B. cereus (% EC24 = 47.5). All the isolates' cell-free extracts exhibited β-hemolysis. The potential of these autochthonous bacterial communities would be explored in the future as depressants for waxy crude oil treatment and for in the oil and gas sector, microbial enhanced oil recovery. VL - 9 IS - 2 ER -
Department of Petroleum Engineering, University of Uyo, Uyo, Nigeria
Department of Petroleum Engineering, University of Uyo, Uyo, Nigeria
Department of Petroleum Engineering, University of Uyo, Uyo, Nigeria
Department of Microbiology, University of Uyo, Uyo, Nigeria
Department of Microbiology, University of Uyo, Uyo, Nigeria
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