Polyporus umbellatus is a kind of medicine and food fungus, in addition to the main component of polysaccharides, steroid components are rich in content, good biological activity and small side effects, part of its efficacy has been widely used in the treatment of various diseases. To investigate the anti-tumor biological activity and pharmacological effects of the steroid components, thiazole blue (MTT) method was used to evaluate the anti-proliferative effect of Polyporus umbellatus steroids on human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line Hela, human renal clear-cell carcinoma cell line 786-O and human renal proximal tubular epithelial cell line HKC. The results indicated that different steroid compounds at different concentrations showed different degrees of inhibition of cell proliferation, as the concentration of the compound increases, its inhibition rate gradually increases, and it is concentration-dependent. The inhibitory effect of different compounds on the proliferation of the same cell was also different, among which the IC50 value of compound ZT-5 on HepG2 cells, Hela cells and 786-O cells was only 14.36 μg/mL, 21.90 μg/mL and 46.56 μg/mL, far lower than that of other compounds. The cytotoxic activity of Polyporus umbellatus steroids against tumor cells HepG2 and Hela was higher than that of 786-O cells, and the cytotoxic activity against normal cells HKC was less, showing obvious cell selectivity. The Polyporus umbellatus steroids have obvious anti-proliferative effect on tumor cells, which expand the therapeutic potential and have important guiding significance for the clinical application of Polyporus umbellatus.
| Published in | Science Discovery (Volume 10, Issue 3) |
| DOI | 10.11648/j.sd.20221003.17 |
| Page(s) | 125-131 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
MTT Method, Polyporus umbellatus, Steroids, Anti-tumor
| [1] | Tan XL, Guo L, Wang GH. Polyporus umbellatus inhibited tumor cell proliferation and promoted tumor cell apoptosis by down-regulating AKT in breast cancer [J]. Biomed Pharmacother, 2016, 83:526-535. |
| [2] | Zeng X, Li CX, Huang Y, et al. Effects of Polyporus umbellatus and Polyporus Polysaccharide on the phagocytosis function and costimulatory molecules expression of peritoneal macrophages in rat bladder cancer [J]. Chin J Immun, 2011, 27(5): 414-418. [曾星,李彩霞, 黄羽,等.猪苓及猪苓多糖对膀胱癌模型大鼠腹腔巨噬细胞吞噬和表面免疫相关分子表达的影响[J].中国免疫学杂志, 2011, 27 (5): 414-418.] |
| [3] | Ye M, Liu ZQ, Wang LJ, et al. Apoptotic effects of polyporusterone A on estrogen receptor negative breast cancer cells via regulating Bcl-2/Bax pathway [J]. Chin Trad Herb Drugs, 2018, 49(22): 5334-5338. [叶敏,刘志强,王李俊,等. 猪苓酮A通过调节Bcl-2/Bax信号通路诱导雌激素受体阴性的乳腺癌细胞凋亡研究[J].中草药, 2018, 49 (22): 5334-5338.] |
| [4] | Zhou WW, Lin WH, Guo SX. Two new polyporusterones isolated from the sclerotia of Polyporus umbellatus [J]. Chem Pharm Bull (Tokyo), 2007, 55(8): 1148-1150. |
| [5] | Sun Y, Yasukawa K. New anti-inflammatory ergostane-type ecdysteroids from the sclerotium of Polyporus umbellatus [J]. Bioorg Med Chem Lett. 2008, 18(11): 3417-3420. |
| [6] | He PF, Zhang AQ, Wang XL, et al. Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia [J]. Int J Biol Macromol, 2016, 82: 411-417. |
| [7] | Sun Y, Zhou X. Purification, initial characterization and immune activities of polysaccharides from the fungus, Polyporus umbellatus [J]. Food Science and Human Wellness, 2014, 3 (2): 73-78. |
| [8] | Liu XL, Xu Y, Zhang YQ, et al. Pharmacology of polyporus polysaccharide against alcoholic fatty liver in rats by lowering blood lipid [J]. Chin Trad Patent Med, 2013, 35(8): 1760-1764. [刘祥兰,徐颖,张钰泉,等.猪苓多糖降血脂抗大鼠酒精性脂肪肝的药理实验研究[J]. 中成药, 2013, 35 (8): 1760-1764.] |
| [9] | Park HG, Lee TH, Chang F, et al. Synthesis of ergosterol and 5,6-dihydroergosterol glycosides and their inhibitory activities on lipopolysaccharide-Induced nitric oxide production [J]. Bull Korean Chem Soc, 2013, 34 (5): 1339-1344. |
| [10] | Zhao YY, Chao X, Zhang Y, et al. Cytotoxic steroids from Polyporus umbellatus [J]. Planta Medica, 2010, 76 (15): 1755-1758. |
| [11] | Zhao YY. Traditional uses, phytochemistry, pharmacology, pharmacokinetics and quality control of Polyporus umbellatus (Pers.) Fries: a review [J]. J Ethnopharmacol. 2013, 149 (1): 35-48. |
| [12] | Pi ZM. Immunology and immunological detection technology [M]. Beijing: Higher Education Press, 2005. [皮至明,免疫学及免疫学检测技术[M].北京:高等教育出版社, 2005.] |
| [13] | Kim TH, Kim JS, Kim ZH, et al. Khz (fusion product of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis in human colon carcinoma HCT116 cells, accompanied by an increase in reactive oxygen species, activation of caspase 3, and increased intracellular Ca2+ [J]. J Med Food, 2015, 18 (3): 332-336. |
| [14] | Chen XM, Tian LX, Guo SX. Research progress on chemical constituents and pharmacological effects of sclerotia of Polyporus umbellatus[J]. Mycosystema, 2017, 36(1): 35-47. [陈晓梅,田丽霞,郭顺星.猪苓化学成分及药理活性研究进展[J].菌物学报, 2017, 36(1): 35-47.] |
| [15] | Liu RJ, Zhang Y, Tian W, et al. Study on the hygroscopic effect of polyporus umbellata and its pharmacological activity[J]. Edible Fungi of China, 2019, 38 (1): 68-71. [刘瑞娟,张叶,田伟,刘亮.猪苓的利水渗湿作用及其药理活性研究[J].中国食用菌, 2019, 38 (1): 68-71.] |
| [16] | Liu HQ, Guo YQ, Xiao P, et al. Research and application of Polyporus umbellata[J]. Guangzhou chemical industry, 2010, 38(10): 40-41. [刘汉卿,郭勇全,肖萍,等.猪苓的研究与应用[J].广州化工,2010, 38 (10): 40-41.] |
| [17] | Liang XN, Zhang XS，Teng HL. The anti-tumor effect Chinese medicine and its active ingredient [J]. Lishizhen Med Mater Med Res, 2013, 24(1): 119-122. [梁欣娜,张兴燊，滕红丽.中药及其有效成分抗肿瘤作用研究[J].时珍国医国药，2013, 24 (1): 119-122.] |
| [18] | Yang Y, Hu WF. MTT method for detecting the anti-tumor effect of compound dachoumu jiangzi soft capsule [J]. J of China Three Gorges Univ (Natural Sciences), 2017, 39 (6): 15-17. [杨燕,胡万福.MTT法检测复方大果木姜子软胶囊抗肿瘤作用[J].三峡大学学报（自然科学版）, 2017, 39 (6): 15-17.] |
| [19] | Sekiya N, Hikiami H, Nakai Y, et al. Inhibitory Effects of Triterpenes Isolated from Chuling on Free Radical-Induced Lysis of Red Blood Cells [J]. Biol Pharm Bull, 2005, 28 (5): 817-821. |
| [20] | Fan HJ, Liang Y, Jiang B, et al. Curcumin inhibits intracellular fatty acid synthase and induces apoptosis in human breast cancer MDA-MB-231 cells [J]. Oncol Rep, 2016, 35 (5): 2651-2656. |
APA Style
Zhang Hai-xiang, Hong Dong-feng, Zhao Xiang-rong, Wang Jin-ping, Guo Hua. (2022). MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids. Science Discovery, 10(3), 125-131. https://doi.org/10.11648/j.sd.20221003.17
ACS Style
Zhang Hai-xiang; Hong Dong-feng; Zhao Xiang-rong; Wang Jin-ping; Guo Hua. MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids. Sci. Discov. 2022, 10(3), 125-131. doi: 10.11648/j.sd.20221003.17
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.sd.20221003.17,
author = {Zhang Hai-xiang and Hong Dong-feng and Zhao Xiang-rong and Wang Jin-ping and Guo Hua},
title = {MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids},
journal = {Science Discovery},
volume = {10},
number = {3},
pages = {125-131},
doi = {10.11648/j.sd.20221003.17},
url = {https://doi.org/10.11648/j.sd.20221003.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20221003.17},
abstract = {Polyporus umbellatus is a kind of medicine and food fungus, in addition to the main component of polysaccharides, steroid components are rich in content, good biological activity and small side effects, part of its efficacy has been widely used in the treatment of various diseases. To investigate the anti-tumor biological activity and pharmacological effects of the steroid components, thiazole blue (MTT) method was used to evaluate the anti-proliferative effect of Polyporus umbellatus steroids on human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line Hela, human renal clear-cell carcinoma cell line 786-O and human renal proximal tubular epithelial cell line HKC. The results indicated that different steroid compounds at different concentrations showed different degrees of inhibition of cell proliferation, as the concentration of the compound increases, its inhibition rate gradually increases, and it is concentration-dependent. The inhibitory effect of different compounds on the proliferation of the same cell was also different, among which the IC50 value of compound ZT-5 on HepG2 cells, Hela cells and 786-O cells was only 14.36 μg/mL, 21.90 μg/mL and 46.56 μg/mL, far lower than that of other compounds. The cytotoxic activity of Polyporus umbellatus steroids against tumor cells HepG2 and Hela was higher than that of 786-O cells, and the cytotoxic activity against normal cells HKC was less, showing obvious cell selectivity. The Polyporus umbellatus steroids have obvious anti-proliferative effect on tumor cells, which expand the therapeutic potential and have important guiding significance for the clinical application of Polyporus umbellatus.},
year = {2022}
}
TY - JOUR T1 - MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids AU - Zhang Hai-xiang AU - Hong Dong-feng AU - Zhao Xiang-rong AU - Wang Jin-ping AU - Guo Hua Y1 - 2022/06/01 PY - 2022 N1 - https://doi.org/10.11648/j.sd.20221003.17 DO - 10.11648/j.sd.20221003.17 T2 - Science Discovery JF - Science Discovery JO - Science Discovery SP - 125 EP - 131 PB - Science Publishing Group SN - 2331-0650 UR - https://doi.org/10.11648/j.sd.20221003.17 AB - Polyporus umbellatus is a kind of medicine and food fungus, in addition to the main component of polysaccharides, steroid components are rich in content, good biological activity and small side effects, part of its efficacy has been widely used in the treatment of various diseases. To investigate the anti-tumor biological activity and pharmacological effects of the steroid components, thiazole blue (MTT) method was used to evaluate the anti-proliferative effect of Polyporus umbellatus steroids on human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line Hela, human renal clear-cell carcinoma cell line 786-O and human renal proximal tubular epithelial cell line HKC. The results indicated that different steroid compounds at different concentrations showed different degrees of inhibition of cell proliferation, as the concentration of the compound increases, its inhibition rate gradually increases, and it is concentration-dependent. The inhibitory effect of different compounds on the proliferation of the same cell was also different, among which the IC50 value of compound ZT-5 on HepG2 cells, Hela cells and 786-O cells was only 14.36 μg/mL, 21.90 μg/mL and 46.56 μg/mL, far lower than that of other compounds. The cytotoxic activity of Polyporus umbellatus steroids against tumor cells HepG2 and Hela was higher than that of 786-O cells, and the cytotoxic activity against normal cells HKC was less, showing obvious cell selectivity. The Polyporus umbellatus steroids have obvious anti-proliferative effect on tumor cells, which expand the therapeutic potential and have important guiding significance for the clinical application of Polyporus umbellatus. VL - 10 IS - 3 ER -
Information
Email: