In Côte d'Ivoire, potatoes have become a key component of the local diet. To meet the rising demand, the government invests over 22 billion FCFA annually to ensure sufficient supply for the population. Despite its favorable climate, Côte d'Ivoire produces very little potatoes. The main constraint to potato cultivation is the lack of seed availability at the right time for producers. This study aims to develop a protocol for producing potato in vitro plantlets in Côte d'Ivoire. Nodal segments from 21-day-old in vitro plantlets were placed onto four basal media containing different combinations of growth regulators (MS0: MS + Morel and Wetmore vitamins; M1: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP; M2: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP+ 0.01 mg/L IAA; M3: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP+ 0.01 mg/L IAA + 0.25mg/L GA3). A series of subcultures was carried out on the medium expressing the best responses. The MS0 medium, lacking growth regulators, supported the highest seedling growth (11.33 cm) and healthy leaf development in all varieties. The Charlotte variety, during its fourth subculture, demonstrated the greatest multiplication capacity, producing plants with an average height of 9.21 cm, 12.1 nodes per leafy shoot, and 3.21 leafy shoots per explant. Beyond this stage, multiplication performance decreases sharply. These results will contribute to the development of a protocol for mass production of potato seeds in the form of in vitro plantlets.
| Published in | American Journal of BioScience (Volume 14, Issue 4) |
| DOI | 10.11648/j.ajbio.20261404.12 |
| Page(s) | 96-104 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2026. Published by Science Publishing Group |
Propagation, Subcultures, Growth Regulators, Potato, Micropropagation
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APA Style
Traoré, P., Touré, Y., Ouattara, B., Togbo, P. E., Koné, T., et al. (2026). Micropropagation Potential of Roseval and Charlotte Varieties of Potato (Solanum Tuberosum L) from Nodal Segments During Subcultures. American Journal of BioScience, 14(4), 96-104. https://doi.org/10.11648/j.ajbio.20261404.12
ACS Style
Traoré, P.; Touré, Y.; Ouattara, B.; Togbo, P. E.; Koné, T., et al. Micropropagation Potential of Roseval and Charlotte Varieties of Potato (Solanum Tuberosum L) from Nodal Segments During Subcultures. Am. J. BioScience 2026, 14(4), 96-104. doi: 10.11648/j.ajbio.20261404.12
AMA Style
Traoré P, Touré Y, Ouattara B, Togbo PE, Koné T, et al. Micropropagation Potential of Roseval and Charlotte Varieties of Potato (Solanum Tuberosum L) from Nodal Segments During Subcultures. Am J BioScience. 2026;14(4):96-104. doi: 10.11648/j.ajbio.20261404.12
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Download@article{10.11648/j.ajbio.20261404.12,
author = {Pyminnan Traoré and Yaya Touré and Brahima Ouattara and Pauleroux Elodie Togbo and Tchoa Koné and Mongomaké Koné},
title = {Micropropagation Potential of Roseval and Charlotte Varieties of Potato (Solanum Tuberosum L) from Nodal Segments During Subcultures},
journal = {American Journal of BioScience},
volume = {14},
number = {4},
pages = {96-104},
doi = {10.11648/j.ajbio.20261404.12},
url = {https://doi.org/10.11648/j.ajbio.20261404.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20261404.12},
abstract = {In Côte d'Ivoire, potatoes have become a key component of the local diet. To meet the rising demand, the government invests over 22 billion FCFA annually to ensure sufficient supply for the population. Despite its favorable climate, Côte d'Ivoire produces very little potatoes. The main constraint to potato cultivation is the lack of seed availability at the right time for producers. This study aims to develop a protocol for producing potato in vitro plantlets in Côte d'Ivoire. Nodal segments from 21-day-old in vitro plantlets were placed onto four basal media containing different combinations of growth regulators (MS0: MS + Morel and Wetmore vitamins; M1: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP; M2: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP+ 0.01 mg/L IAA; M3: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP+ 0.01 mg/L IAA + 0.25mg/L GA3). A series of subcultures was carried out on the medium expressing the best responses. The MS0 medium, lacking growth regulators, supported the highest seedling growth (11.33 cm) and healthy leaf development in all varieties. The Charlotte variety, during its fourth subculture, demonstrated the greatest multiplication capacity, producing plants with an average height of 9.21 cm, 12.1 nodes per leafy shoot, and 3.21 leafy shoots per explant. Beyond this stage, multiplication performance decreases sharply. These results will contribute to the development of a protocol for mass production of potato seeds in the form of in vitro plantlets.},
year = {2026}
}
TY - JOUR T1 - Micropropagation Potential of Roseval and Charlotte Varieties of Potato (Solanum Tuberosum L) from Nodal Segments During Subcultures AU - Pyminnan Traoré AU - Yaya Touré AU - Brahima Ouattara AU - Pauleroux Elodie Togbo AU - Tchoa Koné AU - Mongomaké Koné Y1 - 2026/09/20 PY - 2026 N1 - https://doi.org/10.11648/j.ajbio.20261404.12 DO - 10.11648/j.ajbio.20261404.12 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 96 EP - 104 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20261404.12 AB - In Côte d'Ivoire, potatoes have become a key component of the local diet. To meet the rising demand, the government invests over 22 billion FCFA annually to ensure sufficient supply for the population. Despite its favorable climate, Côte d'Ivoire produces very little potatoes. The main constraint to potato cultivation is the lack of seed availability at the right time for producers. This study aims to develop a protocol for producing potato in vitro plantlets in Côte d'Ivoire. Nodal segments from 21-day-old in vitro plantlets were placed onto four basal media containing different combinations of growth regulators (MS0: MS + Morel and Wetmore vitamins; M1: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP; M2: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP+ 0.01 mg/L IAA; M3: MS + Morel and Wetmore vitamins + 0.25 mg/L BAP+ 0.01 mg/L IAA + 0.25mg/L GA3). A series of subcultures was carried out on the medium expressing the best responses. The MS0 medium, lacking growth regulators, supported the highest seedling growth (11.33 cm) and healthy leaf development in all varieties. The Charlotte variety, during its fourth subculture, demonstrated the greatest multiplication capacity, producing plants with an average height of 9.21 cm, 12.1 nodes per leafy shoot, and 3.21 leafy shoots per explant. Beyond this stage, multiplication performance decreases sharply. These results will contribute to the development of a protocol for mass production of potato seeds in the form of in vitro plantlets. VL - 14 IS - 4 ER -
Faculty of Natural Sciences, Nangui Abrogoua University, Abidjan, Côte d’Ivoire
Faculty of Agricultural, Forestry and Environmental Engineering, University of Man, Man, Côte d'Ivoire
Faculty of Agricultural, Forestry and Environmental Engineering, University of Man, Man, Côte d'Ivoire
Faculty of Natural Sciences, Nangui Abrogoua University, Abidjan, Côte d’Ivoire
Faculty of Natural Sciences, Nangui Abrogoua University, Abidjan, Côte d’Ivoire
Faculty of Natural Sciences, Nangui Abrogoua University, Abidjan, Côte d’Ivoire
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