Research Article
Genetic Diversity and Clonal Dissemination of
ESBL-Producing Klebsiella pneumoniae from Clinical, Food, and Animal Sources in Bouaké, Côte d’Ivoire
Issue:
Volume 14, Issue 5, October 2026
Pages:
105-110
Received:
13 August 2026
Accepted:
31 August 2026
Published:
9 October 2026
DOI:
10.11648/j.ajbio.20261405.11
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Abstract: Klebsiella pneumoniae is a major opportunistic pathogen responsible for multidrug-resistant infections in both hospital and community settings. The emergence and spread of extended-spectrum β-lactamase (ESBL)-producing strains have become a major public health concern due to limited treatment options and their potential spread among human, animal, and food reservoirs. This study aimed to assess the genetic relatedness of ESBL-producing K. pneumoniae isolates collected from human clinical samples, ready-to-eat foods, and animal sources using ERIC-PCR profiling. A total of 98 ESBL-producing K. pneumoniae isolates were included, comprising 96 clinical isolates from hospitalized (n = 91) and non-hospitalized (n = 5) patients at Bouaké University Hospital, one isolate from ready-to-eat food, and one isolate from raw cow’s milk. Identification was performed using conventional bacteriological methods, and ESBL production was confirmed using CHROMagar ESBL medium and the double-disk synergy test. ERIC-PCR analysis revealed a high level of genetic diversity, with 25 distinct ERIC profiles identified, including nine common types and 16 unique types. Several dominant clonal lineages were observed among the clinical isolates, distributed across different hospital departments and outpatient settings. One foodborne isolate shared an ERIC profile identical to that of three clinical isolates, while the animal-derived isolate exhibited a unique genotype. Overall, most isolates showed genetic heterogeneity, although clonal dissemination was observed among hospital strains. These results indicate a predominance of ESBL-producing K. pneumoniae in the hospital setting and suggest limited genetic relatedness between human and non-human isolates. However, the interpretation of relationships involving food and animal isolates remains limited by their low representation in the study. These results underscore the importance of strengthening infection prevention and control measures and confirm the need for ongoing molecular surveillance of multidrug-resistant K. pneumoniae.
Abstract: Klebsiella pneumoniae is a major opportunistic pathogen responsible for multidrug-resistant infections in both hospital and community settings. The emergence and spread of extended-spectrum β-lactamase (ESBL)-producing strains have become a major public health concern due to limited treatment options and their potential spread among human, animal, ...
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Research Article
Storage Insect Pests and Their Effects on Cereal and Pulse Crops in Southern Ethiopia: A Review
Daniel Abebe Geletu*
,
Firew Meka Mentso
Issue:
Volume 14, Issue 5, October 2026
Pages:
111-117
Received:
10 September 2026
Accepted:
22 September 2026
Published:
9 October 2026
DOI:
10.11648/j.ajbio.20261405.12
Downloads:
Views:
Abstract: Storage insect pests are important causes of post-harvest losses in cereal and pulse crops in Southern Ethiopia. Smallholder farmers commonly store harvested grain for several months using traditional storage structures and locally available materials, which may favor insect infestation and population development. This review synthesizes available evidence on major storage insect pests affecting cereal and pulse crops in Southern Ethiopia, their occurrence, damage, and effects on grain quantity, quality, food security, and farmers’ livelihoods. Literature was compiled from scientific databases and other relevant sources, with emphasis on studies conducted in Southern Ethiopia and Ethiopia. Important pests of stored cereals include maize weevil (Sitophilus zeamais), rice weevil (Sitophilus oryzae), lesser grain borer (Rhyzopertha dominica), larger grain borer (Prostephanus truncatus), and Angoumois grain moth (Sitotroga cerealella), while pulse crops are particularly vulnerable to bruchids such as Acanthoscelides obtectus and Callosobruchus spp. Infestation causes grain weight loss, kernel damage, reduced germination, contamination, nutritional deterioration, and reduced market value. Pest occurrence and damage are influenced by crop type, grain moisture, temperature, storage duration, storage structure, hygiene, and initial infestation. Management options include improved storage, sanitation, hermetic storage, botanical materials, inert dusts, and appropriate insecticides. Integrated storage pest management and farmer participation are essential for reducing post-harvest losses and improving food security in Southern Ethiopia
Abstract: Storage insect pests are important causes of post-harvest losses in cereal and pulse crops in Southern Ethiopia. Smallholder farmers commonly store harvested grain for several months using traditional storage structures and locally available materials, which may favor insect infestation and population development. This review synthesizes available ...
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