Major source of drinking water in the Patna region is ground water. This study focuses on assessing the impact of Municipal solid waste on ground water quality near around dumping site area in the Ramchak-Bairiya, Patna, Bihar, India. Dumping sites are in a risk zone of spilling of leachate through adjacent soil which leads to contaminated ground water. Surrounding area of dumping site can be a serious threat to the human health and environment. Huge quantity of municipal solid waste (MSW) dumps containing hazardous materials are a serious threat to the surrounding population of Patna. When these MSW are dumped at site, ground water began contaminating due to percolation of leachate to the porous ground surface. This leachate contains large number of contaminants which can pollute ground water. Ground water samples of different location adjacent to dumping sites were analyzed for microbiological, biochemical characterization, most probable number estimation and antibiotic sensitivity testing of isolated bacteria. The result of MPN test reveals heavy bacterial load in several sampling site which clearly indicates bacterial contamination inside source ground water. Presence of coliform confirms leachate infiltration into the ground water. Antibiotic susceptibility testing demonstrates a pattern of resistance against selective antibiotics. This may suggest the evolve of multidrug resistance (MDR) bacteria in contaminated ground water. Present investigation concludes that leachate of MSW from the Ramchak-Bairiya MSW dumping site severely contaminate the adjacent ground water quality, leading to serious Public health and environmental risks.
| Published in | Frontiers in Environmental Microbiology (Volume 12, Issue 2) |
| DOI | 10.11648/j.fem.20261202.11 |
| Page(s) | 16-22 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2026. Published by Science Publishing Group |
Municipal Solid Waste, Leachate, Ground Water, Coliform, Multi Drug Resistance, Most Probable Number, Bacteriological
S. No. | Abbreviation | Antibiotics | Concentration in mcg |
|---|---|---|---|
1 | A/S | Ampicillin/Sulbactam | 10/10 |
2 | C | Chloramphenicol | 30 |
3 | CIP | Ciprofloxacin | 5 |
4 | E | Erythromycin | 15 |
5 | GEN | Gentamicin | 10 |
6 | LF | Levofloxacin | 5 |
7 | OF | Ofloxacin | 5 |
8 | TE | Tetracycline | 30 |
S. No. | Sample | MPN Index | Total Coliform |
|---|---|---|---|
1 | BI01 | 1-3-1 | 10 |
2 | BI02 | 4-0-3 | 25 |
3 | BI03 | 5-0-1 | 31 |
4 | BI04 | 4-1-1 | 21 |
5 | BI05 | 3-2-0 | 14 |
6 | BI06 | 4-2-3 | 38 |
7 | BI07 | 4-3-1 | 33 |
8 | BI08 | 4-5-1 | 48 |
9 | BI09 | 3-5-0 | 25 |
10 | BI10 | 4-2-2 | 32 |
11 | BI11 | 3-2-0 | 14 |
12 | BI12 | 3-5-0 | 25 |
13 | BI13 | 5-4-0 | 130 |
14 | BI14 | 5-4-2 | 220 |
15 | BI15 | 5-2-4 | 150 |
16 | BI16 | 3-5-0 | 25 |
17 | BI17 | 3-2-0 | 14 |
18 | BI18 | 5-0-3 | 58 |
19 | BI19 | 5-4-3 | 280 |
20 | BI20 | 5-3-4 | 210 |
21 | BI21 | 3-3-2 | 24 |
22 | BI22 | 5-2-0 | 49 |
23 | BI23 | 4-0-2 | 21 |
24 | BI24 | 5-0-1 | 31 |
25 | BI25 | 4-5-1 | 48 |
S. No. | Sample | Indole | MR | VP | Citrate | Oxidase | Urease | Catalase | Probable Bacteria |
|---|---|---|---|---|---|---|---|---|---|
1 | SWA1 | +ve | +ve | +ve | +ve | +ve | +ve | +ve | Aeromonas spp. |
2 | SWA2 | +ve | -ve | +ve | +ve | +ve | +ve | +ve | Aeromonas spp. |
3 | SWA3 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
4 | SWA4 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
5 | SWA5 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
6 | SWA6 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
7 | SWA7 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
8 | SWA8 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
9 | SWA9 | +ve | +ve | -ve | -ve | +ve | +ve | +ve | Aeromonas spp. |
10 | SWA10 | +ve | +ve | -ve | +ve | -ve | Delay | +ve | Citrobacter koseri |
11 | SWA11 | +ve | +ve | -ve | -ve | -ve | +ve | +ve | Escherichia coli |
12 | SWA12 | +ve | +ve | -ve | +ve | -ve | Delay | +ve | Citrobacter koseri |
13 | SWA13 | +ve | +ve | -ve | -ve | -ve | +ve | +ve | Escherichia coli |
14 | SWA14 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
15 | SWA15 | +ve | +ve | -ve | +ve | -ve | +ve | +ve | Proteus spp. |
16 | SWA16 | +ve | +ve | +ve | -ve | -ve | -ve | +ve | Escherichia coli |
17 | SWA17 | +ve | +ve | +ve | -ve | -ve | -ve | +ve | Escherichia coli |
18 | SWA18 | +ve | +ve | -ve | -ve | -ve | +ve | +ve | Escherichia coli |
19 | SWA19 | +ve | +ve | -ve | -ve | -ve | +ve | +ve | Escherichia coli |
20 | SWA20 | +ve | +ve | -ve | -ve | -ve | +ve | +ve | Escherichia coli |
21 | SWA21 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
22 | SWA22 | +ve | +ve | -ve | -ve | -ve | +ve | +ve | Escherichia coli |
23 | SWA23 | +ve | +ve | -ve | +ve | +ve | +ve | +ve | Aeromonas spp. |
24 | SWA24 | +ve | +ve | -ve | -ve | -ve | +ve | +ve | Escherichia coli |
25 | SWA25 | +ve | +ve | -ve | -ve | +ve | Delay | +ve | Aeromonas spp. |
26 | SWA26 | +ve | +ve | -ve | +ve | +ve | +ve | +ve | Aeromonas spp. |
27 | SWA27 | +ve | -ve | +ve | +ve | -ve | +ve | +ve | Klebsiella oxytoca |
S. No. | Sample | AMP | C | CIP | E | GEN | LE | OF | TE |
|---|---|---|---|---|---|---|---|---|---|
1 | SWA1 | 12 | 12 | 30 | 20 | 30 | 22 | 11 | 6 |
2 | SWA2 | 12 | 28 | 34 | 13 | 35 | 26 | 22 | 22 |
3 | SWA3 | 2 | 24 | 40 | 22 | 42 | 28 | 25 | 24 |
4 | SWA4 | 17 | 18 | 34 | 22 | 25 | 24 | 22 | 17 |
5 | SWA5 | 16 | 17 | 35 | 18 | 36 | 24 | 24 | 24 |
6 | SWA6 | 17 | 25 | 30 | 22 | 30 | 23 | 15 | 17 |
7 | SWA7 | 15 | 22 | 33 | 16 | 40 | 26 | 22 | 17 |
8 | SWA8 | 6 | 18 | 31 | 8 | 45 | 22 | 22 | 6 |
9 | SWA9 | 16 | 22 | 34 | 16 | 30 | 25 | 22 | 22 |
10 | SWA10 | 17 | 15 | 30 | 14 | 35 | 22 | 19 | 22 |
11 | SWA11 | 6 | 20 | 35 | 15 | 32 | 23 | 17 | 6 |
12 | SWA12 | 18 | 32 | 37 | 17 | 31 | 25 | 22 | 24 |
13 | SWA13 | 6 | 16 | 33 | 18 | 30 | 20 | 19 | 21 |
14 | SWA14 | 6 | 12 | 25 | 13 | 30 | 17 | 18 | 13 |
15 | SWA15 | 20 | 20 | 32 | 13 | 20 | 24 | 21 | 24 |
16 | SWA16 | 16 | 27 | 35 | 10 | 6 | 20 | 16 | 20 |
17 | SWA17 | 16 | 22 | 32 | 27 | 40 | 22 | 22 | 24 |
18 | SWA18 | 20 | 20 | 30 | 17 | 40 | 22 | 22 | 22 |
19 | SWA19 | 17 | 24 | 32 | 21 | 40 | 24 | 22 | 22 |
20 | SWA20 | 15 | 28 | 28 | 17 | 35 | 24 | 22 | 24 |
21 | SWA21 | 17 | 22 | 25 | 12 | 42 | 21 | 17 | 19 |
22 | SWA22 | 16 | 20 | 30 | 20 | 31 | 22 | 18 | 20 |
23 | SWA23 | 16 | 16 | 28 | 12 | 34 | 20 | 17 | 22 |
24 | SWA24 | 19 | 16 | 30 | 11 | 31 | 20 | 18 | 15 |
25 | SWA25 | 17 | 28 | 30 | 20 | 34 | 20 | 16 | 22 |
26 | SWA26 | 6 | 6 | 26 | 15 | 36 | 20 | 15 | 30 |
27 | SWA27 | 15 | 29 | 34 | 16 | 37 | 23 | 15 | 32 |
MSW | Municipal Solid Waste |
MDR | Multidrug Resistance |
MPN | Most Probable Number |
Mcg | Microgram |
MAR | Multiple Antibiotic Resistance |
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APA Style
Bharti, S., Singh, S. K., Shreyansh, Anand, A., Mishra, P. (2026). Impact of Municipal Solid Waste Landfill Leachate on Groundwater Quality: A Case Study of Ramchak-Bairiya, Patna, Bihar. Frontiers in Environmental Microbiology, 12(2), 16-22. https://doi.org/10.11648/j.fem.20261202.11
ACS Style
Bharti, S.; Singh, S. K.; Shreyansh; Anand, A.; Mishra, P. Impact of Municipal Solid Waste Landfill Leachate on Groundwater Quality: A Case Study of Ramchak-Bairiya, Patna, Bihar. Front. Environ. Microbiol. 2026, 12(2), 16-22. doi: 10.11648/j.fem.20261202.11
@article{10.11648/j.fem.20261202.11,
author = {Sweta Bharti and Sushil Kumar Singh and Shreyansh and Aparna Anand and Prem Mishra},
title = {Impact of Municipal Solid Waste Landfill Leachate on Groundwater Quality: A Case Study of Ramchak-Bairiya, Patna, Bihar},
journal = {Frontiers in Environmental Microbiology},
volume = {12},
number = {2},
pages = {16-22},
doi = {10.11648/j.fem.20261202.11},
url = {https://doi.org/10.11648/j.fem.20261202.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20261202.11},
abstract = {Major source of drinking water in the Patna region is ground water. This study focuses on assessing the impact of Municipal solid waste on ground water quality near around dumping site area in the Ramchak-Bairiya, Patna, Bihar, India. Dumping sites are in a risk zone of spilling of leachate through adjacent soil which leads to contaminated ground water. Surrounding area of dumping site can be a serious threat to the human health and environment. Huge quantity of municipal solid waste (MSW) dumps containing hazardous materials are a serious threat to the surrounding population of Patna. When these MSW are dumped at site, ground water began contaminating due to percolation of leachate to the porous ground surface. This leachate contains large number of contaminants which can pollute ground water. Ground water samples of different location adjacent to dumping sites were analyzed for microbiological, biochemical characterization, most probable number estimation and antibiotic sensitivity testing of isolated bacteria. The result of MPN test reveals heavy bacterial load in several sampling site which clearly indicates bacterial contamination inside source ground water. Presence of coliform confirms leachate infiltration into the ground water. Antibiotic susceptibility testing demonstrates a pattern of resistance against selective antibiotics. This may suggest the evolve of multidrug resistance (MDR) bacteria in contaminated ground water. Present investigation concludes that leachate of MSW from the Ramchak-Bairiya MSW dumping site severely contaminate the adjacent ground water quality, leading to serious Public health and environmental risks.},
year = {2026}
}
TY - JOUR T1 - Impact of Municipal Solid Waste Landfill Leachate on Groundwater Quality: A Case Study of Ramchak-Bairiya, Patna, Bihar AU - Sweta Bharti AU - Sushil Kumar Singh AU - Shreyansh AU - Aparna Anand AU - Prem Mishra Y1 - 2026/07/17 PY - 2026 N1 - https://doi.org/10.11648/j.fem.20261202.11 DO - 10.11648/j.fem.20261202.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 16 EP - 22 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20261202.11 AB - Major source of drinking water in the Patna region is ground water. This study focuses on assessing the impact of Municipal solid waste on ground water quality near around dumping site area in the Ramchak-Bairiya, Patna, Bihar, India. Dumping sites are in a risk zone of spilling of leachate through adjacent soil which leads to contaminated ground water. Surrounding area of dumping site can be a serious threat to the human health and environment. Huge quantity of municipal solid waste (MSW) dumps containing hazardous materials are a serious threat to the surrounding population of Patna. When these MSW are dumped at site, ground water began contaminating due to percolation of leachate to the porous ground surface. This leachate contains large number of contaminants which can pollute ground water. Ground water samples of different location adjacent to dumping sites were analyzed for microbiological, biochemical characterization, most probable number estimation and antibiotic sensitivity testing of isolated bacteria. The result of MPN test reveals heavy bacterial load in several sampling site which clearly indicates bacterial contamination inside source ground water. Presence of coliform confirms leachate infiltration into the ground water. Antibiotic susceptibility testing demonstrates a pattern of resistance against selective antibiotics. This may suggest the evolve of multidrug resistance (MDR) bacteria in contaminated ground water. Present investigation concludes that leachate of MSW from the Ramchak-Bairiya MSW dumping site severely contaminate the adjacent ground water quality, leading to serious Public health and environmental risks. VL - 12 IS - 2 ER -