Research Article
Anti-Staphylococcus Aureus Guide Isolation of Compounds from the Fruits of Ziziphus Mauritiana
Aboubakar Ali Mahamat*,
Haroun Ali Adannou
,
Haroun Heloua,
Paul Etoga Mbarga,
Ouahouo Wache Blandine,
Pierre Mkounga,
Ephrem Augustin Nkengfack
Issue:
Volume 14, Issue 1, February 2026
Pages:
1-10
Received:
8 December 2025
Accepted:
12 January 2026
Published:
30 January 2026
Abstract: The present work deals with the isolation, purification and characterization of secondary metabolites of the fruits of Ziziphus mauritiana and the evaluation of their biological activity against Staphylococcus aureus. A plant from the Rhamnaceae family, widely used in the Cameroonian and Tchadian traditional pharmacopoeia for the treatment of various diseases such as food poisoning, pneumonia, urinary tract infections, and skin infections such as wounds and ulcers. Our investigations focused on the methanol/dichloromethane (MeOH/DCM, 1:1) extract of the fruits of Ziziphus mauritiana. Alkaloidal treatment was performed on this extract, and we obtained four (04) fractions: two non-alkaloid fractions, F1 (DCM), F2 (EtOAc), and two alkaloid fractions, F3 (DCM) and F4 (EtOAc). Using usual chromatographic methods (CC, CCM), we isolated from the alkaloidal fraction F3, two compounds 1 and 2, and four compounds 3, 4, 5, 6 from the non-alkaloid fraction F1, the hexane fraction. From these compounds, three (03) were fully characterized using spectroscopic methods 1H and 13C NMR (1 and 2 dimensions) and by comparison of their spectral data with those of the literature. These are: Sanjoinenine 1, betulinic acid 3, and epicatechin 4. The crude extract as well as fraction, and some isolated compounds were evaluated in vitro by the liquid microdilution method described by CLSI, against two strains of Staphylococcus aureus: S. aureus ATCC25923, S. aureus ATCC43300, and a Clinical isolate. The crude extract, fractions F1 and F4, were the most active fractions tested against the strain S. aureus ATCC 25923, with a MIC of 15.6, 62.5, and 15.6 µg/mL, respectively, while the other fractions (F2 and F3) showed moderate activity against the same strain. Fraction F2 is not active. For the S. aureus ATCC 43300, all the fractions have a weak activity, and for the clinical isolate, only ZSA shows a good activity with a MIC of 62.5 µg/mL, and the others show a moderate activity.
Abstract: The present work deals with the isolation, purification and characterization of secondary metabolites of the fruits of Ziziphus mauritiana and the evaluation of their biological activity against Staphylococcus aureus. A plant from the Rhamnaceae family, widely used in the Cameroonian and Tchadian traditional pharmacopoeia for the treatment of vario...
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Research Article
Fatty Acid Profiles and Physicochemical Properties of Kernels from Four Senegalese Varieties of
Mangifera indica L
Samba Fama Ndoye,
Lalla Aicha Ba,
Dan Eugene Bernardi,
Mbaye Diaw Dioum,
Insa Seck,
Seydou Ka,
Ismaila Ciss,
Abda Ba,
Seynabou Sokhna,
Moussa Ndao,
Illa Tea,
Matar Seck*
Issue:
Volume 14, Issue 1, February 2026
Pages:
11-17
Received:
27 February 2026
Accepted:
12 March 2026
Published:
26 March 2026
DOI:
10.11648/j.ajac.20261401.12
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Views:
Abstract: The mango (Mangifera indica), belonging to the genus Mangifera of the family Anacardiaceae, is cultivated worldwide as an economically important fruit crop. It is also widely used in traditional medicine, which has stimulated growing interest in investigating and evaluating the therapeutic potential of its bioactive compounds. This study was carried out to assess physicochemical parameters and fatty acid composition of Mangifera indica seed oil collected from four Senegalese varieties. The oil contents obtained for the four varieties were 7.08%, 7.5%, 7.3 and 7.11% respectively for Sewe, Bouka, Palmer and Dieg bou gate. The oils, which were characterized by Gas Chromatography (GC) with Flame Ionization Detector (FID), were mainly composed of stearic acid, ranging from 41.849 ± 0.048 to 47.793 ± 0.071%, and oleic acid from 31.085 ± 0.153 to 39.053 ± 0.050%. Among the four Senegalese mango cultivars, Palmer has the highest oleic acid content (39.053 ± 0.050%). Significant amounts of palmitic, linoleic, and arachidonic acids were also detected, while the remaining fatty acids in the mango kernel were present only in trace amounts. The saponification values were identified to be in the range of 212.9 - 234.2 mg KOH/g of oil, and the iodine values ranging from 50.4 to 59.7 g I2 /100 g of oil. The study showed that oils from different Senegalese cultivars of Mangifera indica have potential for various industrial applications.
Abstract: The mango (Mangifera indica), belonging to the genus Mangifera of the family Anacardiaceae, is cultivated worldwide as an economically important fruit crop. It is also widely used in traditional medicine, which has stimulated growing interest in investigating and evaluating the therapeutic potential of its bioactive compounds. This study was carrie...
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