Research Article
Effect of Bacillus Megaterium 2333, Lactobacillus Casei 76 and Their Combination as a Probiotic Supplement Feed on the Growth Performance of Piglets
Chang-Gon Sin*,
Won-Ju Hwang,
Chang-Su Kim,
Hak-Chol Choe,
Su-Chol Rim*,
Hyok-Won Kim,
Chon-Il Kim,
Kwang-Il To*
,
Jong-Chol Son
Issue:
Volume 13, Issue 3, September 2025
Pages:
38-43
Received:
18 April 2025
Accepted:
3 June 2025
Published:
10 July 2025
Abstract: To feed piglets during the weaning period is critical in swine production farming. Probiotics could be alternative to antibiotics to reduce suckling stress and improve growth performance. The aim of this study was to investigate the effect of supplemental feed manufactured by Bacillus megaterium 2333, Lactobacillus casei 76 and their combination on the growth performance of suckling piglets. First, the endurance to harsh environment and adhesion ability were tested to evaluate the possibility of probiotic use for these two bacteria. Then, supplemental feed was manufactured by addition of chosen bacteria. Feed composition was chemically analyzed. Four groups of piglets, namely Control, Bacillus megaterium 2333, Lactobacillus casei 76 and their combination group were created to compare the effect. The result showed that supplemental feed manufactured by probiotic addition exerted beneficial effect on the growth performance of suckling and weaned piglets regardless of nutritional value of feed. Incidence of diarrhea and the number of pathogen such as E. coli and Salmonella were also lower in probiotic group. Especially, combination use of Bacillus megaterium 2333 and Lactobacillus casei 76 showed the best result of lowest feed conversion ratio. The result of present study indicated that the supplemental feed manufactured by the combination of Bacillus megaterium 2333 and Lactobacillus casei 76 was beneficial to rearing piglets.
Abstract: To feed piglets during the weaning period is critical in swine production farming. Probiotics could be alternative to antibiotics to reduce suckling stress and improve growth performance. The aim of this study was to investigate the effect of supplemental feed manufactured by Bacillus megaterium 2333, Lactobacillus casei 76 and their combination on...
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Research Article
Effect of Seed Spray Treatment by DCPTA on Plant Growth and Yield of Soybean (Glycine max L.)
Jong-U Ri,
Kwang-Il To*,
Chung-Il Ri,
Sok-Chol Ri,
Hung-Man Jong,
Son-Hyok Jo,
Su-Chol Rim
Issue:
Volume 13, Issue 3, September 2025
Pages:
44-50
Received:
26 May 2025
Accepted:
19 June 2025
Published:
12 August 2025
Abstract: DCPTA (2-diethylaminoethyl-3,4-dichlorophenylether) is one of the most representative tertiary compounds and appears to have the potential for biochemical manipulation of plant growth. Here we presented effective spray treatment method to soybean (Glycine max L.) seeds and revealed that DCPTA spray treatment influenced plant growth and increased the yield of soybean. The suitable concentration of DCPTA was 10 mg/L. 60~70 kg of soybean seeds was evenly sprayed with 1 L amount of DCPTA diluent and covered with plastic sheet for 0.5~1 h. After soybean seeds absorbed DCPTA diluent sufficiently, they were dried under the shade condition and sowed. Compared with the control, application of 10 mg/L DCPTA seed spray treatment increased significantly the length of primary shoot and root of germinated seeds, and shoot dry weight, root dry weight, the number of nodules, weight of nodules were enhanced 110.5%, 111.9%, 132.8%, 119.2%, respectively at the vegetative growth stage. During a period between the ending of vegetative growth and the beginning of reproductive growth stage, the height of soybean was decreased and the numbers of branches were increased clearly. DCPTA spray treatment clearly increased the yield of soybeans, especially the highest yield (2475 kg) of seeds was observed in the treatment of 10 mg/L DCPTA compared with the control. The yield of soybean increased to 128%, thus, we confirmed that seed spray treatment by DCPTA is effective, eco-friendly and successful way in order to increase the soybean yield.
Abstract: DCPTA (2-diethylaminoethyl-3,4-dichlorophenylether) is one of the most representative tertiary compounds and appears to have the potential for biochemical manipulation of plant growth. Here we presented effective spray treatment method to soybean (Glycine max L.) seeds and revealed that DCPTA spray treatment influenced plant growth and increased th...
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Review Article
The Role of Molecular Tools in Microalgal Strain Improvement: Current Status and Future Perspectives
Alebachew Molla*,
Gedif Meseret
Issue:
Volume 13, Issue 3, September 2025
Pages:
51-57
Received:
24 July 2025
Accepted:
7 August 2025
Published:
26 August 2025
DOI:
10.11648/j.abb.20251303.13
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Abstract: Microalgae are microscopic, typically single-celled photosynthetic organisms found in freshwater, marine, and even terrestrial environments. Microalgae are crucial to global ecosystems because they are primary producers, forming the base of the aquatic food chain and producing about half of the Earth's oxygen through photosynthesis. Microalgae are vital sustainable feedstocks with applications spanning food, feed, biofuels, and high-value bioproducts. However, their industrial-scale use faces challenges due to the limited robustness and productivity of wild-type strains. Recent advancements in molecular tools and genetic engineering have ushered in a new era for microalgal strain improvement. Molecular tools, including genetic engineering, random mutagenesis, and advanced selection methods such as fluorescence-activated cell sorting (FACS), constitute powerful approaches for microalgal strain improvement. These tools enable precise genome modifications, creation of tailor-made phenotypes, and selection of mutants with enhanced productivity and stress tolerance. The scope of this review encompasses the diverse molecular techniques employed in strain optimization covering forward and reverse genetics, site-directed mutagenesis, adaptive laboratory evolution, and non-GMO random mutagenesis. The significance lies in overcoming bottlenecks in microalgal commercialization by improving strain performance and enabling sustainable bioproduct generation. This article aims to synthesize current advancements, critically analyze the integration of these molecular tools with high-throughput technologies, discuss regulatory considerations, and outline future perspectives for accelerating microalgal strain development to meet industrial and environmental demands.
Abstract: Microalgae are microscopic, typically single-celled photosynthetic organisms found in freshwater, marine, and even terrestrial environments. Microalgae are crucial to global ecosystems because they are primary producers, forming the base of the aquatic food chain and producing about half of the Earth's oxygen through photosynthesis. Microalgae are ...
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