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Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group
Fereydon Rezazadeh,
Neda Hosseinzadeh,
Nahid Ghaderian
Issue:
Volume 4, Issue 6, November 2016
Pages:
86-92
Received:
1 October 2016
Accepted:
11 October 2016
Published:
3 November 2016
Abstract: Biomarkers are biological gnomons of processes or events occurring within the body. Biomarkers can be sub-divided into leakage enzymes (Cardiac troponin I) and functional markers (Natriuretic peptides). Some studies were represented that these biomarkers may be changes in respiratory disorders. Therefore, present study is performed to evaluate serumic levels of cardiac biomarkers between horses with respiratory disease and apparently healthy horses. Initially 10 horses with manifestations of respiratory disease (include increasing of respiratory and heart rate, coughing, mucopurulent and purulent nasal discharge, mucosal hyperemia, increased CRT) and 8 apparently healthy horses underwent a clinical examination. Age, gender, breed and color of individual horses were recorded. CTnI and NT pro BNP were measured by chemiluminescence immunoassay but ANP was measured by ELISA kit in the serum of blood. Electrocardiogram (ECG) has been recorded in base-apex lead in the stall. The mean ages were of 2.1±00.8 and 1.7±0.8 in apparently healthy horses and patient groups, respectively. The mean value level of all cardiac biomarkers (cTnI, ANP and NT pro BNP) in patient group (0.008±0.002 ng/ml, 280.48±79.51 ng/L and 20.34±0.68 pg/ml) was higher than apparently healthy group (0.007±0.002 ng/ml, 206.92±23.01 ng/L and 20.00±0.00 pg/ml). But, the statistical differences between the groups were not significant (P>0.05). No significant differences were found in waveforms of electrocardiograms between two groups.
Abstract: Biomarkers are biological gnomons of processes or events occurring within the body. Biomarkers can be sub-divided into leakage enzymes (Cardiac troponin I) and functional markers (Natriuretic peptides). Some studies were represented that these biomarkers may be changes in respiratory disorders. Therefore, present study is performed to evaluate seru...
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The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran
Mohammad Khezri,
Mojtaba Moharami,
Hussein Modirrousta,
Zahra Salahi,
Babak Rokhzad
Issue:
Volume 4, Issue 6, November 2016
Pages:
93-96
Received:
12 July 2016
Accepted:
26 July 2016
Published:
14 December 2016
Abstract: European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.
Abstract: European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (...
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Comparison of Some Hematological Parameters Between Horses in an Endurance Competition
Fereydon Rezazadeh,
Neda Hosseinzadeh,
Raziollah Jafari Jozani,
Nima Dianati
Issue:
Volume 4, Issue 6, November 2016
Pages:
97-102
Received:
13 November 2016
Accepted:
28 November 2016
Published:
6 January 2017
Abstract: This study conducted to comparison of some blood constituents between horses participated an endurance racing. Jugular vein puncture was done to collect blood from15 horses (1 gelding, 2 mares, and 12 male). These horses had been classified to three breeds include 12Kurd, 1 pure Arab, 2cross-breed and participated in the endurance racing of 0 (before competition), 15 (After first loop) and 30 km (final loop). Horses were classified in three age-groups include below 5(n=1), 5-10 (n=10) and above 10years of age (n=4). Hematocrit and Hemoglobin percentage, Total protein concentration, Red blood cell and White blood cell counting, glucose and cortisol level were measured at before starting competition (0), 15 and 30 km. Data was analyzed by SPSS (version 22). Results showed significant difference in glucose level after 30 km (P<0.05) but not after 15km (P>0.05). No significant difference was observed between cortisol concentration and age groups (P>0.05) but, there was significant difference between cortisol concentration and gender (P<0.05). Breed had influenced on HT, Hb, TP and WBC (P<0.05). Otherwise breed had no effect on RBC, glucose level and cortisol concentration (P>0.05). HT and WBC counting were higher in Arab, cross breed and Kurd respectively. Hemoglobin percentage was found higher in Arab, Kurd and Cross breed respectively. Total protein concentration in Arab was higher than Cross breed and Kurd. In conclusion, in short distance endurance competition of horses, stress is not effect on metabolism of animal, furthermore, gender and breed could important in blood parameters alterations.
Abstract: This study conducted to comparison of some blood constituents between horses participated an endurance racing. Jugular vein puncture was done to collect blood from15 horses (1 gelding, 2 mares, and 12 male). These horses had been classified to three breeds include 12Kurd, 1 pure Arab, 2cross-breed and participated in the endurance racing of 0 (befo...
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Meat-Borne Parasites a Health Hazard Concern in the Sudan: A Review
Ghada Hassan Abdelnabi,
Shawgi Mohamed Hassan,
Atif Elamin Abdelgadir,
Elgailani Ali Elamin
Issue:
Volume 4, Issue 6, November 2016
Pages:
103-107
Received:
1 December 2016
Accepted:
19 December 2016
Published:
21 January 2017
Abstract: Food-borne diseases in general have received more attention in the last decade, but little attention has been paid to parasitic food-borne infections. This is probably due to the fact that they are not associated with acute illness as bacterial and viral infections do. In the Sudan, the most important parasitic meat-borne infections are Taenia saginata, Toxoplasma gondii, Sarcocystis spp., Linguatula serrata and fish infection with trematode metacercaria. Control measures used in the country to prevent infection with these parasites are through inspecting meat in slaughterhouses for cysticercosis. Toxoplasma and Sarcocystsis infections are not considered during routine meat inspection due to lack of techniques for detection of these infections. Prevalence of infection with these parasites in humans and livestock in all States of Sudan is not available. Methods for routine diagnosis, monitoring or recording of these infections are inadequate, or not existing, in most of the laboratories. Studies are required to establish seroprevalence in livestock and humans. There is an urgent need to monitor and control meat-borne parasites using new technologies such as serological and molecular techniques, health education and vaccination. Researchers are urged to participate and establish innovative ways and means to control these diseases.
Abstract: Food-borne diseases in general have received more attention in the last decade, but little attention has been paid to parasitic food-borne infections. This is probably due to the fact that they are not associated with acute illness as bacterial and viral infections do. In the Sudan, the most important parasitic meat-borne infections are Taenia sagi...
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